Use of a low-density immiscible compound in a device for detecting an analyte in a sample
The present disclosure relates to a device for detecting an analyte in a sample, and especially for detecting antigen/antibody reactions, wherein said device comprises a layer composed of a low-density immiscible compound. The disclosure also relates to a method for detecting an analyte in a sample and especially for detecting antigen/antibody reactions in a device comprising a layer composed of a low-density immiscible compound. The disclosure further relates to the uses of such low-density immiscible compound.
1. A device for detecting an analyte in a sample, the device comprising:
a reaction chamber able to receive the sample;
a separation matrix; and
a layer composed of a low-density immiscible compound that separates the reaction chamber from the separation matrix,
wherein the reaction chamber is located above the separation matrix within the device.
2. The device according to claim 1 , wherein the separation matrix and/or the reaction chamber comprises an analyte ligand.
3. The device according to claim 2 , wherein the analyte ligand is an antibody, an antibody fragment, or an antigen.
4. The device according to claim 1 , wherein the low-density immiscible compound has a lower density than the density of the separation matrix.
5. The device according to claim 1 , wherein the low-density immiscible compound has a density of less than 1, and/or the low-density immiscible compound is hydrophobic.
6. The device according to claim 1 , wherein the low-density immiscible compound is a composition comprising one or more compounds selected from: synthetic oil, organic oil, mineral oil, paraffinic oil, paraffin, non-polar solvents, fatty acids, an alkane mixture and a pure alkane.
7. The device according to claim 1 , wherein the low-density immiscible compound is liquid or solid at room temperature.
8. A method for detecting the presence of an analyte in a sample, the method comprising:
a) providing a device according to claim 1 ;
b) dispensing the sample into the reaction chamber of the device;
c) exposing the device to sedimentation by gravitation and/or centrifugation;
wherein reagents comprising an analyte ligand are provided at least at step a) and/or at step b); and
identifying the presence of the analyte in the sample based on the formation of an analyte-ligand complex on or within the separation matrix after sedimentation, or
identifying the absence of the analyte in the sample based on the lack of formation of the analyte-ligand complex on or within the separation matrix after sedimentation.
9. The method according to claim 8 , wherein the low-density immiscible compound has a lower density than the density of the separation matrix.
10. The method according to claim 8 , wherein the low-density immiscible compound is liquid or solid at room temperature.
11. The method according to claim 10 , wherein the low-density immiscible compound is solid and the solid compound is liquefied thermally or chemically.
12. A kit for detecting an analyte in a sample, the kit comprising:
a device comprising:
a reaction chamber able to receive the sample;
a separation matrix; and
a layer composed of a low-density immiscible compound that separates the reaction chamber from the separation matrix,
wherein the reaction chamber is located above the separation matrix within the device.