Method of improving liquid sample flow in assay device
Disclosed is method of improving flow of a liquid sample in an assay device, as well as a method of performing an assay on a liquid sample for the detection of one or more analytes of interest. The methods use the addition of a reagent, such as a wash reagent, to wet the fluid flow path of a sample prior to sample addition, thereby improving flow of the sample through the fluid flow path that has been wetted as opposed to flow of the sample through the fluid flow path that has not been wetted. The reagent wets the fluid flow path between the sample addition zone and the wicking zone.
1. An assay device comprising:
a substrate;
a liquid sample addition zone disposed on the substrate;
a reagent zone downstream and in fluid communication with the sample addition zone and containing a reagent material;
a detection zone in fluid communication with the reagent zone;
a wicking zone in fluid communication with the detection zone having a capacity to receive liquid sample flowing from the detection zone, wherein the sample addition zone, the reagent zone, the detection zone and the wicking zone defining a fluid flow path; and
a reagent addition zone along and in fluid communication with the fluid flow path downstream of the sample addition zone and upstream of the detection zone in which at least a portion of the fluid flow path includes a plurality of adjacent projections, the plurality of projections having a height, width and distance or distances between the projections such that lateral capillary flow is achieved,
wherein a reagent is applied to the reagent addition zone, the reagent having a volume that is less than a volume of the liquid sample, and
wherein the plurality of projections, the positioning of the reagent addition zone and the volume of the applied reagent provide for the applied reagent to flow upstream along the fluid flow path toward the sample addition zone and flow downstream along the fluid flow path toward the wicking zone, wetting the fluid flow path to create wettability of the fluid flow path in advance of the addition of the liquid sample to the sample addition area of the device.
2. The assay device as claimed in claim 1 , in which the entire fluid flow path includes the plurality of projections.
3. The assay device as claimed in claim 2 , in which at least some of the projections are given a biological, chemical or physical functionality.
4. The assay device as claimed in claim 3 , in which capture elements are attached to the projections in the detection zone of the device.
5. The assay device as claimed in claim 1 , wherein the applied reagent includes a wash reagent and the liquid sample includes whole blood.
6. An assay device comprising:
a substrate;
a liquid sample addition zone disposed on the substrate;
a reagent zone downstream and in fluid communication with the sample addition zone and containing a reagent material;
a detection zone in fluid communication with the reagent zone;
a wicking zone in fluid communication with the detection zone having a capacity to receive liquid sample flowing from the detection zone, wherein the sample addition zone, the reagent zone, the detection zone and the wicking zone defining a fluid flow path; and
a reagent addition zone along and in fluid communication with the fluid flow path downstream of the sample addition zone and upstream of the detection zone,
wherein a reagent is applied to the reagent addition zone, the reagent having a volume that is less than a volume of the liquid sample, and
wherein the positioning of the reagent addition zone and the volume of the applied reagent provide for the applied reagent to flow upstream along the fluid flow path toward the sample addition zone and flow downstream along the fluid flow path toward the wicking zone to create wettability of the fluid flow path in advance of the addition of the liquid sample to the sample addition area of the device.
7. The assay device as claimed in claim 6 , further comprising a plurality of adjacent projections located in at least a portion of the fluid flow path upstream and downstream of the reagent addition zone.
8. The assay device as claimed in claim 7 , wherein the plurality of projections include a height, width, and distance or distances between the projections such that lateral capillary flow is achieved.
9. The assay device as claimed in claim 7 , wherein the plurality of projections provide for capillary flow of the applied reagent to flow upstream toward the sample addition zone and downstream toward the wicking zone to wet the fluid flow path.
10. The assay device as claimed in claim 7 , in which the entire fluid flow path includes the plurality of projections.
11. The assay device as claimed in claim 7 , in which at least some of the projections are given a biological, chemical or physical functionality.
12. The assay device as claimed in claim 7 , in which capture elements are attached to the projections in the detection zone of the device.
13. The assay device as claimed in claim 6 , wherein the applied reagent includes a wash reagent and the liquid sample includes whole blood.