Method for producing nanocelluloses from a cellulose substrate
Disclosed is a method for producing nanocelluloses from a cellulose substrate including cellulose fibers, the method including the following sequence of steps: —a step of enzymatic treatment of the cellulose substrate, by bringing same into contact with at least one cleaving enzyme, then—a step of mechanical treatment of the cellulose substrate subjected to the step of enzymatic treatment, in order to delaminate the cellulose fibres and obtain the nanocelluloses. The at least one cleaving enzyme is chosen from the enzymes belonging to the family of lytic polysaccharide monooxygenases (LPMOs) capable of achieving cleavage in the presence of an electron donor. Also disclosed are the nanocelluloses obtained according to the method.
1. A process for producing nanocelluloses from a cellulose-based substrate comprising cellulose fibers, said process comprising the following successive steps:
one or more step(s) of enzymatic treatment of said cellulose-based substrate, by bringing it into contact with at least one cleavage enzyme consisting of enzymes belonging to the lytic polysaccharide monooxygenase (LPMO) family capable of carrying out an oxidative cleavage of said cellulose fibers in the presence of a donor electron,
wherein said at least one LPMO enzyme is purified from a culture supernatant of a fungus and/or produced in a heterologous system,
wherein said at least one LPMO introduces, into the cellulose fibers, charged groups which create electrostatic repulsions, then
at least one step of mechanical treatment of said cellulose-based substrate subjected to said one or more step(s) of enzymatic treatment, wherein said at least one step of mechanical treatment is chosen among mechanical treatments exerting a shear action, in order to delaminate said cellulose fibers and to obtain said nanocelluloses,
wherein said oxidative cleavage of said cellulose fibers, catalyzed by said at least one LPMO, facilitates the delamination of these cellulose fibers during said at least one step of mechanical treatment.
2. The process for producing nanocelluloses as claimed in claim 1 , wherein the LPMOs are chosen from the enzymes capable of carrying out a cleavage of the cellulose by oxidation of at least one of the carbon atoms in positions C 1 , C 4 and C 6 of the glucose ring.
3. The process for producing nanocelluloses as claimed in claim 2 , wherein the LPMOs are chosen from the AA9 and AA10 families of the CAZy classification.
4. The process for producing nanocelluloses as claimed in claim 1 , wherein the LMPOs are chosen from the LPMOs derived from Podospora anserina.
5. The process for producing nanocelluloses as claimed in claim 4 , wherein the LMPOs are chosen from PaLPMO9A (Genbank CAP68375), PaLPMO9B (Genbank CAP73254), PaLPMO9D (Genbank CAP66744), PaLPMO9E (Genbank CAP67740), PaLPMO9F (Genbank CAP71839), PaLPMO9G (Genbank CAP73072) and PaLPMO9H (Genbank CAP61476).
6. The process for producing nanocelluloses as claimed in claim 1 , wherein the electron donor is chosen from ascorbate, gallate, catechol, reduced glutathione, lignin fragments and fungal carbohydrate dehydrogenases.
7. The process for producing nanocelluloses as claimed in claim 1 , wherein the cellulose-based substrate is obtained from wood, a cellulose-rich fibrous plant, beetroot, citrus fruits, annual straw plants, marine animals, algae, fungi or bacteria.
8. The process for producing nanocelluloses as claimed in claim 1 , wherein the cellulose-based substrate is chosen from chemical papermaking pulps.
9. The process for producing nanocelluloses as claimed in claim 8 , wherein the cellulose-based substrate is chosen from chemical wood papermaking pulps.
10. The process for producing nanocelluloses as claimed in claim 9 , wherein the cellulose-based substrate is chosen from at least one of the following chemical wood papermaking pulps:
bleached pulps,
semi-bleached pulps,
raw pulps,
bisulfite pulps,
sulfate pulps,
sodium hydroxide pulps,
kraft pulps.
11. The process for producing nanocelluloses as claimed in claim 1 , wherein, following said at least one step of mechanical treatment, said process comprises a post-treatment step.
12. The process for producing nanocelluloses as claimed in claim 11 , said wherein the process comprises a post-treatment step chosen from an acid treatment, an enzymatic treatment, an oxidation, an acetylation, a silylation, or else a derivatization of certain chemical groups borne by the nanocelluloses.
13. The process for producing nanocelluloses as claimed in claim 1 , wherein the nanocelluloses obtained consist of cellulose nanofibrils and/or of cellulose nanocrystals.
14. The process for producing nanocelluloses as claimed in claim 1 , wherein said at least one step of mechanical treatment, exerting a shear action, comprises at least a homogenization treatment.
15. The process for producing nanocelluloses as claimed in claim 1 , wherein said at least one step of mechanical treatment, exerting a shear action, comprises at least a microfluidization treatment.
16. The process for producing nanocelluloses as claimed in claim 1 , wherein said at least one step of mechanical treatment, exerting a shear action, comprises at least an abrasion treatment.
17. The process for producing nanocelluloses as claimed in claim 1 , wherein said at least one step of mechanical treatment, exerting a shear action, comprises at least a cryomilling treatment.