Patent
This invention relates to methods and compositions for providing a benefit to a plant by associating the plant with a beneficial endophyte of the genus Streptomyces , including benefits to a plant derived from a seed or other plant element treated with said endophyte. For example, this invention provides purified endophytes, synthetic combinations comprising endophytes, and methods of making and using the same. In particular, this invention relates to compositions and methods of improving soybean and maize plants.
1. A method of increasing abundance of one or more arbuscular mycorrhizal fungi in roots of a plant under water-stressed conditions, comprising treating a plant reproductive element with a formulation comprising an effective amount of a Streptomyces endophyte that is heterologous to the plant reproductive element, wherein the Streptomyces endophyte comprises
a 16S polynucleotide sequence having at least 97% identity to SEQ ID NO 2 and at least 3 polynucleotides that encode an arabinose transporter protein;
wherein the effective amount is effective to increase abundance of one or more arbuscular mycorrhizal fungi in roots of a plant grown from the treated reproductive element under water-stressed conditions, as compared to an isoline plant grown from a plant reproductive element not treated with the Streptomyces endophyte.
2. The method of claim 1 , wherein the plant is soybean.
3. The method of claim 1 , wherein the plant reproductive element is a seed.
4. The method of claim 3 , wherein the seed is a transgenic seed.
5. The method of claim 1 , wherein the formulation comprises a purified population of the Streptomyces endophyte at a concentration of at least about 10{circumflex over ( )}2 CFU/ml in a liquid formulation or about 10{circumflex over ( )}2 CFU/gm in a non-liquid formulation.
6. The method of claim 1 , wherein the formulation further comprises one or more of the following: stabilizer, preservative, carrier, surfactant, anticomplex agent, fungicide, nematicide, bactericide, insecticide, herbicide, or any combination thereof.
7. The method of claim 1 , wherein the treating comprises coating the plant reproductive element with the formulation, spraying the formulation onto the plant reproductive element, or introducing the formulation onto a soil comprising the plant reproductive element.
8. The method of claim 1 , wherein the arbuscular mycorrhizal fungi is of the family Glomeraceae.
9. The method of claim 8 , wherein the arbuscular mycorrhizal fungi is of the genera Glomus.
10. The method of claim 1 , wherein the 16S polynucleotide sequence is SEQ ID NO: 2.