Systems and methods for sample use maximization
The present invention provides systems, devices, and methods for point-of-care and/or distributed testing services. The methods and devices of the invention are directed toward automatic detection of analytes in a bodily fluid. The components of the device can be modified to allow for more flexible and robust use with the disclosed methods for a variety of medical, laboratory, and other applications. The systems, devices, and methods of the present invention can allow for effective use of samples by improved sample preparation and analysis.
1. A method of processing a sample, the method comprising:
a) inserting a cartridge into a sample processing device,
wherein the cartridge comprises:
a first assay unit and a second assay unit, wherein the first assay unit and second assay unit are each independently movable from the cartridge, wherein at least the first assay unit has the shape of a pipette tip, wherein the cartridge further comprises reagents for performing a first assay in the first assay unit and for performing a second assay in the second assay unit, and
wherein the sample processing device comprises:
a detection station comprising at least one of a: spectrophotometer, PMT, photodiode, camera, CCD sensor, or CMOS sensor;
a fluid handling system comprising a nozzle configured to interface with a pipette tip and configured to move the sample; and
an imaging device configured to obtain images of said nozzle and said pipette tip,
b) dividing the sample into at least a first portion and a second portion in the sample processing device;
c) retaining the first portion of the sample in a first module and transporting by the fluid handling system the second portion of the sample to the second assay unit,
d) obtaining an image of the nozzle and of the pipette tip within the sample processing device, effective to provide information regarding whether there are undesirable condition(s) associated with the pipette tip; and
e) processing the sample.
2. The method of claim 1 wherein the undesirable condition(s) includes one or more of the following: bubbles in the sample.
3. The method of claim 1 wherein the undesirable condition(s) includes one or more of the following: particles that interfere with measurement of characteristic(s) of the sample.
4. The method of claim 1 wherein the undesirable condition(s) includes one or more of the following: debris that interferes with measurement of characteristic(s) of the sample.
5. The method of claim 1 wherein the undesirable condition(s) includes one or more of the following: precipitates that interfere with measurement of characteristic(s) of the sample.
6. The method of claim 1 wherein the undesirable condition(s) includes one or more of the following: bubbles, particles, fibers, debris, and precipitates that interfere with measurement of characteristic(s) of the sample.
7. The method of claim 1 wherein the pipette tip has a conical, tapered shape.
8. The method of claim 1 , wherein the sample processing device further comprises a centrifuge, and at least a portion of the sample is centrifuged in the centrifuge.
9. The method of claim 1 , wherein the sample processing device transfers information from the detection station to an external device.
10. The method of claim 1 , wherein the sample is blood, and wherein dividing the sample into at least a first portion and a second portion comprises dividing the blood into a plasma portion and a red blood cell-containing portion.
11. The method of claim 10 , wherein the first portion comprises the plasma portion and the second portion comprises the red blood cell-containing portion.