ACELLULAR BIOLOGIC COMPOSITION AND METHOD OF MANUFACTURE
A biological composition has a mixture of mechanically selected allogeneic biologic material derived from bone marrow. The mixture has non-whole cellular components including vesicular components and active and inactive components of biological activity, cell fragments, cellular excretions, cellular derivatives, and extracellular components. The mixture including non-whole cell fractions including one or more of exosomes, transcriptosomes, proteasomes, membrane rafts, lipid rafts. The mixture is compatible with biologic function.
1 . A biological composition comprising:
a mixture of mechanically selected allogeneic biologic material derived from bone marrow having non-whole cellular components including vesicular components and active and inactive components of biological activity, cell fragments, cellular excretions, cellular derivatives, and extracellular components; and
wherein the mixture is compatible with biologic function.
2 . The biological composition of claim 1 further comprises bone particles, the bone particles being added to the mixture derived from bone marrow.
3 . The biological composition of claim 2 wherein the bone particles include a mixture of cortical bone particles and cancellous bone particles.
4 . The biological composition of claim 1 wherein the mixture of mechanically selected material derived from bone marrow further includes a select number of non-whole cell fractions including one or more of exosomes, transcriptosomes, proteasomes, membrane rafts, lipid rafts.
5 . The biological composition of claim 4 wherein the combination of non-whole cell components with a select number of the non-whole cell fractions sustains pluripotency in both graft or host cells or combinations thereof.
6 . The biological composition of claim 5 wherein the select number of the non-whole cell fractions sustains pluripotency in graft or host cells or combinations thereof includes differentiated committed cells and non-differentiated and non-committed cells.
7 . The biological composition of claim 2 wherein the biological composition is predisposed to demonstrate or support elaboration of active volume or spatial geometry consistent in morphology with that of endogenous bone.
8 . The biological composition of claim 1 wherein the biological composition extends regenerative resonance that compliments or mimics tissue complexity.
9 . The biological composition of claim 1 wherein the mixture is treated in a protectant or cryoprotectant prior to preservation or cryopreservation or freeze drying.
10 . The biological composition of claim 9 wherein the protectant or cryoprotectant creates a physical or electrical or chemical gradient or combination thereof for tissue regeneration.
11 . The biological composition of claim 10 wherein the gradient has a physical characteristic of modulus or topography such as charge density, field shape or cryo or chemo toxics tendencies.
12 . The biological composition of claim 10 wherein the gradient has a chemical characteristic of spatially changing compositions of density or species of functional molecules, wherein the molecules can offer a fixed catalytic function as a co-factor.
13 . The biological composition of claim 10 wherein the gradient has an electrical characteristic of charge based or pH based or electron affinities that confer metastability in biologic potential.
14 . The biological composition of claim 4 wherein the bone marrow mixture which is derived from a cadaver has separation-enhanced non-whole cell fractions vitality including one or more of the following: separating the fractions from cells heightens their vitality, reversing “arrest” of donors, responsive molecular coupling, matrix quest in neutralizing inflammation or satience by balancing stimulus for repair.
15 . The biological composition of claim 9 wherein the protectant or cryoprotectant is a polyampholyte.
16 . The biological composition of claim 8 wherein the regenerative resonance occurs in the presence or absence of a refractory response.
17 . The biological composition of claim 9 wherein the cryopreservation occurs at a temperature that is sub-freezing.
18 . The biological composition of claim 17 wherein the cryopreservation temperature is from 0 degrees C. to −200 degrees C.
19 . The biological composition of claim 1 wherein the mixture creates a physical or electrical or chemical gradient or combination thereof for tissue regeneration.
20 . The biological composition of claim 19 wherein the gradient has a physical characteristic such as modulus or topography.
21 . The biological composition of claim 19 wherein the gradient has a chemical characteristic such as spatially changing compositions of density or species of functional molecules.
22 . The biological composition of claim 19 wherein the gradient has an electrical characteristic such as charge based or pH based.
23 . The biological composition of claim 1 can be organelle fragments.
24 . The biological composition of claim 1 wherein active an inactive components of biological activity can be extants of the human metabolome.
25 . The biological composition of claim 9 wherein the composition is maintained at ambient temperature prior to freeze drying.