Bone marrow stem cell compositions, methods of making, and uses thereof

1. A method of making a bone marrow stem cell composition or implant containing viable stem cells, the method comprising:

harvesting bone marrow from a cadaver donor to obtain harvested bone marrow;

heating the harvested bone marrow to about 20° C. to about 40° C.;

selectively lysing the harvested bone marrow to obtain bone marrow stem cells, wherein the step of selectively lysing is performed using water or a solution containing 0.5% or less v/v acetic acid, wherein the step of selective lysing occurs at about 20° C. to about 40° C., and wherein one or more of the bone marrow stem cells are viable.

2. The method of claim 1 , wherein the method further comprises the step of washing the harvested bone marrow prior to selectively lysing the harvested bone marrow.

3. The method of claim 1 , wherein the method further comprises the step of centrifuging after the step of selectively lysing the harvested bone marrow.

4. The method of claim 1 , wherein the method further comprises the step of filtering after the step of selectively lysing the harvested bone marrow.

5. The method of claim 1 , wherein the method further comprises the step of adding a stabilization solution after the step of selectively lyisng the harvested bone marrow.

6. The method of claim 1 , further comprising the step of mixing the bone marrow stem cell composition or implant with a scaffold material after the step of selectively lysing the harvested bone marrow.

7. The method of claim 6 , wherein the scaffold material is allograft bone.

8. The method of claim 1 , further comprising the step of adding a scaffold material after the step of harvesting the bone marrow and before the step of lysing.

9. The method of claim 8 , wherein the scaffold material is allograft bone.

10. The method of claim 6 , wherein at least one of the bone marrow stem cells is attached to the scaffold material.

11. The method of claim 7 , wherein at least one of the bone marrow stem cells is attached to the allograft bone.

12. The method of claim 8 , wherein at least one of the bone marrow stem cells is attached to the scaffold material after the step of lysing.

13. The method of claim 9 , wherein at least one of the bone marrow stem cells is attached to the allograft bone after the step of lysing.

14. The method of claim 1 , further comprising delivering the bone marrow stem cell composition or implant to a subject.

15. The method of claim 6 , further comprising delivering the bone marrow stem cell composition or implant to a subject.

16. The method of claim 7 , further comprising delivering the bone marrow stem cell composition or implant to a subject.

17. The method of claim 8 , further comprising delivering the bone marrow stem cell composition or implant to a subject.

18. The method of claim 9 , further comprising delivering the bone marrow stem cell composition or implant to a subject.

19. The method of claim 1 , wherein the step of heating, the step of lysing, or both the steps of heating and lysing are performed at about 37° C.