Image acquisition apparatus
An image acquisition apparatus including: a stage on which a specimen is mounted; an objective lens that collects light from the specimen; a stage driving part that drives the stage; an image capturing unit that acquires an image by photographing the light collected by the objective lens; an image-generating unit that generates a pasted image by pasting the captured image acquired by the image capturing unit; a storage unit that stores the pasted image; a color-difference calculating unit that calculates a degree of dissimilarity between a color of the pasted image and a color of the captured image prior to pasting; and a color-difference correcting unit that corrects the color of the captured image so as to match the color of the pasted image, on the basis of the calculated degree of dissimilarity.
1. An image acquisition apparatus comprising:
a stage on which a specimen is mounted;
an objective lens that collects light from the specimen mounted on the stage;
an actuator that drives the stage in a direction intersecting an optical axis of the objective lens;
an image sensor that acquires an image by photographing the light collected by the objective lens; and
a processor comprising hardware, the processor being configured to:
generate a pasted image by pasting the acquired image;
store the pasted image in a memory;
calculate a degree of dissimilarity between a color of the pasted image and a color of the acquired image in a region which is shared by the pasted image and the acquired image prior to pasting of the pasted image stored in the memory and the acquired image; and
correct the color of an entire area of the acquired image so as to match the color of the pasted image, on the basis of the calculated degree of dissimilarity.
2. The image acquisition apparatus according to claim 1 , wherein, for a shared region between the pasted image stored in the memory and the acquired image, processor calculates the degree of dissimilarity using a weight that becomes smaller as distance from the center of the shared region becomes greater.
3. The image acquisition apparatus according to claim 1 , wherein, each time the degree of dissimilarity is calculated, the processor cumulatively adds and stores a rounding error generated in the calculation, and when the degree of dissimilarity is calculated, the cumulatively added value of the rounding error of a previous calculation, which is stored, is added.
4. An image acquisition apparatus comprising:
a stage on which a specimen is mounted;
an objective lens that collects light from the specimen mounted on the stage;
an actuator that drives the stage in a direction intersecting an optical axis of the objective lens;
an image sensor that acquires an image by photographing the light collected by the objective lens;
a memory in which a pasted image is stored; and
a processor comprising hardware, the processor being configured to:
calculate a degree of dissimilarity between a color of the pasted image stored in the memory and a color of the acquired image in a region which is shared by the pasted image and the acquired image;
correct the color of an entire area of the acquired image on the basis of the calculated degree of dissimilarity; and
generate a new pasted image by pasting the corrected acquired image on the pasted image and storing a new pasted image in the memory.
5. The image acquisition apparatus according to claim 4 , wherein, for a shared region between the pasted image stored in the memory and the acquired image, the processor calculates the degree of dissimilarity using a weight that becomes smaller as distance from the center of the shared region becomes greater.
6. The image acquisition apparatus according to claim 4 , wherein, each time the degree of dissimilarity is calculated, the processor cumulatively adds and stores a rounding error generated in the calculation, and adds the stored cumulatively added value of the rounding error of a previous calculation in order to calculate the degree of dissimilarity when the degree of dissimilarity is calculated.
7. The image acquisition apparatus according to claim 1 , wherein the processor calculates the degree of dissimilarity as one of a difference or a ratio between a pixel value of each of colors of the pasted image and a pixel value of each of colors of the acquired image in the region which is shared by the pasted image and the acquired image prior to pasting.
8. The image acquisition apparatus according to claim 4 , wherein the processor calculates the degree of dissimilarity as one of a difference or a ratio between a pixel value of each of colors of the pasted image stored in the memory and a pixel value of each of colors of the acquired image in the region which is shared by the pasted image and the acquired image.