Use of gene expression profiling to predict survival in cancer patient
Gene expression profiling in multiple myeloma patients identifies genes that distinguish between patients with subsequent early death or long survival after treatment. Poor survival is linked to over-expression of genes such as ASPM, OPN3 and CKS1B which are located in chromosome 1q. Given the frequent amplification of 1q in many cancers, it is possible that these genes can be used as powerful prognostic markers and therapeutic targets for multiple myeloma and other cancer.
1. A detection method comprising:
obtaining an isolated nucleic acid sample from plasma cells isolated from a multiple myeloma patient; and
measuring gene expression levels of GNG10, PNPLA4, KIAA1754, AHCYL1, MCLC, EVI5, AD-020, PARG1, CTBS, FUCA1, RFP2, FLJ20489, LTBP1, TRIP13, AIM2, SELI, SLC19A1, LARS2, OPN3, ASPM, CCT2, UBE21, SIK6, FLJ13052, FLJ12525, BIRC5, CKS1B, CKAP1, MGC57827, DKFZp7790175, PFN1, ILF3, IF116, TBRG4, PAPD1, EIF2C2, MGC4308, ENO1, DSG2, EXOSC4, 7AGLN2, RUVBL1, ALDOA, CPSF3, MGC15606, LGALS1, RADI8, SNX5, PSMD4, RAN, KIF14, CBX3, TMPO, DKFZP586L0724, WEE1, ROBO1, TCOF1, YWHAZ, and MPHOSPH1 in the plasma cells by contacting the isolated nucleic acid sample with nucleic acid probes that hybridize to each gene and detecting the complex formed between the plasma cell RNA and each probe, wherein the method comprises detecting reduced expression of GNG10, PNPLA4, KIAA1754, AHCYL1, MCLC, EVI5, AD-020, PARG1, CTBS, FUCA1, RFP2, FLJ20489, or LTBP1, relative to a control from a patient having a favorable prognosis, or detecting increased expression of TRIP13, AIM2, SELI, SLC19A1, LARS2, OPN3, ASPM, CCT2, UBE21, STK6, FLJ13052, FLJ12525, BIRC5, CKS1B, CKAP1, MGC57827, DKFZp7790175, PFN1, ILF3, IFI16, TBRG4, PAPD1, EIF2C2, MGC4308, EN1L, DSG2, EXOSC4, TAGLN2, RUVBL1, ALDOA, CPSF3, MGC15606, LGALS1, RAD18, SNX5, PSMD4, RAN, KIF14, CBX3, TMPO, DKFZP586L0724, WEE1, ROBO1, TCOF1, YWHAZ, or MPHOSPH1, relative to a control from a patient having a favorable prognosis.
2. The method of claim 1 , wherein measuring the gene expression levels comprises generating cDNA from the isolated nucleic acid sample isolated from the plasma cells.
3. The method of claim 1 , wherein the patient is undergoing, or previously underwent, treatment comprising high-dose chemotherapy.
4. The method of claim 3 , wherein the high dose chemotherapy comprises bortezomib, thalidomide, or a combination thereof.
5. The method of claim 1 , wherein the patient is undergoing, or previously underwent, treatment comprising autologous peripheral blood stem cell transplantation.
6. The method of claim 1 , wherein the nucleic acid sample is artificially and detectably labeled.
7. The method of claim 6 , wherein the gene expression levels are measured by detecting a complex between the artificially and detectably labeled nucleic acid sample and a synthetic microarray.
8. The method of claim 1 , wherein the plasma cells are CD138+ plasma cells.