IP Library › Granted Patent US 10,036,051
Granted Patent B2
US 10,036,051 · App. 15/594,031 · Granted Jul 31, 2018

Enhanced processive cellulases

Inventors: William S. Adney (Pittsboro, NC); Gregg T. Beckham (Golden, CO); Eric Jarvis (Boulder, CO); Michael E. Himmel (Littleton, CO); Stephen R. Decker (Berthoud, CO); Jeffrey G. Linger (Denver, CO); Kara Podkaminer (Boulder, CO); John O. Baker (Golden, CO); Larry Taylor, II (Berthoud, CO); Qi Xu (Lakewood, CO); Arjun Singh (Lakewood, CO)
Assignee: Alliance for Sustainable Energy, LLC
C12P19/14C10L1/02C12N9/2437C12P7/10C12Y302/01004C12Y302/01021C10L2290/26
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 10,036,051
App. No.
15/594,031
Granted
Jul 31, 2018
Kind
B2
Abstract

Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. These nucleic acids may be expressed in hosts such as fungi, which in turn may be cultured to produce chimeric polypeptides. Also disclosed are chimeric polypeptides and their use in the degradation of cellulosic materials.

Claims (19)

1. An isolated chimeric fungal Cel7A polypeptide that has a cellulase activity at least 1.5-fold greater than the wild-type Cel7A polypeptide, comprising a catalytic domain (CD), a carbohydrate-binding molecule (CBM) and a linker domain, wherein at least one of the domains is from a Penicillium funiculosum Cel7A polypeptide and at least one of the domains is from a Trichoderma reesei Cel7A polypeptide; and wherein each domain is from the Penicillium funiculosum or Trichoderma reesei Cel7A polypeptides.

2. The isolated chimeric fungal Cel7A polypeptide of claim 1 , wherein the chimeric fungal Cel7A polypeptide has a cellulase activity of at least 2-fold greater than the wild-type Cel7A polypeptide.

3. The isolated chimeric fungal Cel7A polypeptide of claim 1 , wherein the chimeric fungal Cel7A polypeptide comprises the catalytic domain (CD) from the Penicillium funiculosum Cel7A polypeptide.

4. The isolated chimeric fungal Cel7A polypeptide of claim 3 , wherein the chimeric fungal Cel7A polypeptide further comprises the carbohydrate-binding molecule (CBM) from the Trichoderma reesei Cel7A peptide.

5. The isolated chimeric fungal Cel7A polypeptide of claim 4 , wherein the chimeric fungal Cel7A polypeptide further comprises the linker domain from the Penicillium funiculosum Cel7A polypeptide.

6. The isolated chimeric fungal Cel7A polypeptide of claim 4 , wherein the chimeric fungal Cel7A polypeptide comprises the linker domain from the Trichoderma reesei Cel7A polypeptide.

7. The isolated chimeric fungal Cel7A polypeptide of claim 3 , wherein the chimeric fungal Cel7A polypeptide further comprises the linker domain from the Trichoderma reesei Cel7A polypeptide.

8. The isolated chimeric fungal Cel7A polypeptide of claim 7 , wherein the chimeric fungal Cel7A polypeptide further comprises the carbohydrate-binding molecule (CBM) from the Penicillium funiculosum Cel7A polypeptide.

9. The isolated chimeric fungal Cel7A polypeptide of claim 1 , wherein the chimeric fungal Cel7A polypeptide comprises the catalytic domain (CD) from the Trichoderma reesei Cel7A polypeptide.

10. The isolated chimeric fungal Cel7A polypeptide of claim 9 , wherein the chimeric fungal Cel7A polypeptide further comprises the linker domain from the Penicillium funiculosum Cel7A polypeptide.

11. The isolated chimeric fungal Cel7A polypeptide of claim 10 , wherein the chimeric fungal Cel7A polypeptide further comprises the carbohydrate-binding molecule (CBM) form the Penicillium funiculosum Cel7A polypeptide.

12. The isolated chimeric fungal Cel7A polypeptide of claim 10 , wherein the chimeric fungal Cel7A polypeptide further comprises the carbohydrate-binding molecule (CBM) from the Trichoderma reesei Cel7A polypeptide.

13. The isolated chimeric fungal Cel7A polypeptide of claim 9 , wherein the chimeric fungal Cel7a polypeptide further comprises the carbohydrate-binding molecule (CBM) from the Penicillium funiculosum Cel7A polypeptide.

14. The isolated chimeric fungal Cel7A polypeptide of claim 13 , wherein the chimeric fungal Cel7A polypeptide further comprises the linker domain from the Trichoderma reesei Cel7A polypeptide.

15. A method for degrading cellulose or lignocellulose biomass, comprising contacting the cellulose or lignocellulosic biomass with the isolated chimeric Cel7A polypeptide according to claim 1 .

16. A method for producing a biofuel from lignocellulosic biomass, comprising:

a) contacting the lignocellulosic biomass with an enzyme cocktail comprising the isolated chimeric fungal Cel7A polypeptide according to claim 1 to generate sugars; and

b) converting the sugars to a biofuel by fermentation.

17. The method of claim 16 , wherein the enzyme cocktail further comprises an endoglucanase, a β-glucosidase, or both.

Assignments (3)
CHANGE OF NAME Recorded Dec 16, 2025
From: ALLIANCE FOR SUSTAINABLE ENERGY, LLC
To: ALLIANCE FOR ENERGY INNOVATION, LLC
Reel/Frame 073993/0276 →
CONFIRMATORY LICENSE Recorded Feb 8, 2018
From: NATIONAL RENEWABLE ENERGY LABORATORY
To: UNITED STATES DEPARTMENT OF ENERGY
Reel/Frame 045292/0230 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 12, 2017
From: ADNEY, WILLIAM S.; BECKHAM, GREGG T.; JARVIS, ERIC; HIMMEL, MICHAEL E.; DECKER, STEPHEN R.; LINGER, JEFFREY G.; PODKAMINER, KARA; BAKER, JOHN O.; TAYLOR, LARRY, II; XU, QI; SINGH, ARJUN
To: ALLIANCE FOR SUSTAINABLE ENERGY, LLC
Reel/Frame 042357/0639 →
Continuity (3)
Division 14365200
Provisional Application 61576585 · Dec 16, 2011
Related Publication 20170247731A1 · Aug 31, 2017