Chemical engineering processes and apparatus for the synthesis of compounds
The present invention provides methods for producing cannabinoids and cannabinoid analogs as well as a system for producing these compounds. The inventive method is directed to contacting a compound according to Formula I or Formula II with a cannabinoid synthase. Also described is a system for producing cannabinoids and cannabinoid analogs by contacting a THCA synthase with a cannabinoid precursor and modifying at least one property of the reaction mixture to influence the quantity formed of a first cannabinoid relative to the quantity formed of a second cannabinoid.
1. A bioreactor for producing cannabidiolic acid (CBDA) and cannabichromenic acid (CBCA), wherein said bioreactor comprises: cannabigerolic acid (CBGA) and CBDA synthase; and wherein said bioreactor comprises a control mechanism configured to control conditions in the bioreactor to modify the ratio of CBDA to CBCA so produced wherein the control mechanism comprises addition of one or more organic solvents selected from the group consisting of dimethyl sulfoxide (DMSO), dimethyl formamide (DMF), and isopropoyl alcohol; wherein the concentration of said solvent is maintained between 10% and 40% (v/v).
2. The bioreactor of claim 1 , wherein the bioreactor further comprises a yeast cell genetically engineered to express the CBDA synthase which is capable of producing CBDA and CBCA from the CBGA.
3. A bioreactor for producing cannabidiolic acid (CBDA) and cannabichromenic acid (CBCA), wherein said bioreactor comprises: cannabigerolic acid (CBGA) and CBDA synthase; and wherein said bioreactor comprises a control mechanism configured to control conditions in the bioreactor to modify the ratio of CBDA to CBCA so produced wherein the mechanism to control said conditions comprises addition of cyclodextrin as a solvent wherein the concentration of said cyclodextrin is maintained to between 0.2% (w/v) and 2.8% (w/v).