Methods using FTIR for plant trait detection and trait introgression
Provided are methods and/or systems having advantages of cost effective, time saving, and informative user-friendly characteristics to accomplish trait introgression. The methods provided comprise determining presence of omega-3 fatty acids (for example docosahexaenoic acid or DHA; docosapentaenoic acid or DPA; Alpha linolenic acid or ALA; and eicosapentaenoic acid or EPA) using Fourier Transformed infra Red (FTIR) spectrum. The use of FTIR enables analysis of the oil contained in the seeds using a multivariate-based Mid-FTIR model. The methods and/or systems provided advantages of non-destructive analysis to provide information to facilitate trait introgression and other breeding applications.
1. A method for determining presence of docosahexaenoic acid (DHA) in a viable whole plant seed, the method comprising
(a) obtaining one or more viable whole plant seeds;
(b) performing a Fourier Transform Infrared (FTIR) spectroscopy analysis of each of the one or more viable whole plant seeds, wherein the spectroscopy analysis comprises use of a detector cooled using liquid nitrogen to detect absorbance at a first absorption band between about 3800 cm −1 wavenumber and about 2600 cm −1 wavenumber;
(c) comparing the absorbance of step (b) to existing reference FTIR absorbance;
(d) selecting at least one of the one or more viable whole plant seeds analyzed in step (b) based on the detected absorbance of step (b), wherein each selected seed is viable and predicted to contain DHA based on the detected absorbance of step (b); and
(e) planting or breeding with the at least one seed selected in step (d).
2. The method of claim 1 , further comprising using the detector to detect absorbance at a second absorption band between about 7400 cm −1 wavenumber and about 5400 cm −1 wavenumber.
3. The method of claim 1 , wherein the one or more viable whole plant seeds obtained in (a) are from a plant selected from the group consisting of canola, soybean, sunflower, cotton, Brassica , rapeseed, peanut, corn, wheat, rice, alfalfa, and oat.
4. The method of claim 1 , wherein the one or more viable whole plant seeds obtained in (a) are Brassica napus seeds.
5. The method of claim 1 , wherein the one or more viable whole plant seeds obtained in (a) are from a transgenic plant.