IP Library Granted Patent US 10,407,740
Granted Patent B2
US 10,407,740 · App. 15/601,712 · Granted Sep 10, 2019

Oligonucleotides for use in determining the presence of

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Quick Facts
Patent No.
US 10,407,740
App. No.
15/601,712
Granted
Sep 10, 2019
Kind
B2
Abstract

Oligomers useful for determining the presence of Trichomonas vaginalis in a test sample.

Claims (22)

1. A first capture probe consisting of: (i) a target-complementary sequence, the base sequence of which is selected from the group consisting of SEQ ID NO:90, SEQ ID NO:91, SEQ ID NO:95, SEQ ID NO:96, and an RNA/DNA combination equivalent of any of the foregoing; and (ii) a non-complementary sequence comprising a homopolymer tail capable of stably hybridizing, under assay conditions, to a complementary sequence in an oligonucleotide that is bound to a solid support, wherein the non-complementary sequence does not comprise a sequence capable of stably hybridizing to Trichomonas vaginalis derived nucleic acid under assay conditions.

2. The first capture probe of claim 1 , wherein the non-complementary sequence comprises a spacer region for joining the target-complementary sequence to the homopolymer tail.

3. The first capture probe of claim 1 , wherein the homopolymer tail is a poly dA sequence, and wherein the complementary sequence in the oligonucleotide bound to the solid support is a poly dT sequence.

4. The first capture probe of claim 3 , wherein the non-complementary sequence comprises a spacer region for joining the target-complementary sequence to the homopolymer tail, and wherein the spacer region is a poly dT sequence.

5. The first capture probe of claim 1 , wherein the target-complementary sequence of the first capture probe comprises a ribonucleoside having 2′-O-methyl substitution to the ribofuranosyl moiety.

6. A set of capture probes comprising:

the first capture probe of claim 1 ; and

a second capture probe consisting of: (i) a target-complementary sequence, the base sequence of which is selected from the group consisting of SEQ ID NO:55, SEQ ID NO:56, SEQ ID NO:57, SEQ ID NO:58, and an RNA/DNA combination equivalent of any of the foregoing; and (ii) a non-complementary sequence comprising a homopolymer tail capable of stably hybridizing, under assay conditions, to a complementary sequence in an oligonucleotide that is bound to a solid support, wherein the non-complementary sequence does not comprise a sequence capable of stably hybridizing to Trichomonas vaginalis derived nucleic acid under assay conditions.

7. The set of capture probes of claim 6 , wherein the non-complementary sequence comprises a spacer region for joining the target-complementary sequence to the homopolymer tail.

8. The set of capture probes of claim 6 , wherein the homopolymer tail is a poly dA sequence, and wherein the complementary sequence in the oligonucleotide bound to the solid support is a poly dT sequence.

9. The set of capture probes of claim 8 , wherein the non-complementary sequence comprises a spacer region for joining the target-complementary sequence to the homopolymer tail, and wherein the spacer region is a poly dT sequence.

10. The set of capture probes of claim 6 , wherein the target-complementary sequence of at least one of the first and second capture probes comprises a ribonucleoside having 2′-O-methyl substitution to the ribofuranosyl moiety.

11. The first capture probe of claim 1 , wherein the base sequence of the target-complementary sequence consists of the base sequence of SEQ ID NO:90, SEQ ID NO:91, or an RNA/DNA combination equivalent thereof.

12. The first capture probe of claim 11 , wherein the base sequence of the first capture probe consists of the base sequence of SEQ ID NO:94 or SEQ ID NO:105.

13. The set of capture probes of claim 6 , wherein the base sequence of the first capture probe target-complementary sequence consists of the base sequence of SEQ ID NO:90, SEQ ID NO:91, or an RNA/DNA combination equivalent thereof.

14. The set of capture probes of claim 13 , wherein the base sequence of the first capture probe consists of the base sequence of SEQ ID NO:94 or SEQ ID NO:105.

15. The set of capture probes of claim 6 , wherein the base sequence of the second capture probe target-complementary sequence consists of the base sequence of SEQ ID NO:55, SEQ ID NO:56, or an RNA/DNA combination equivalent thereof.

16. The set of capture probes of claim 15 , wherein the base sequence of the second capture probe consists of the base sequence of SEQ ID NO:59.

17. The set of capture probes of claim 13 , wherein the base sequence of the second capture probe target-complementary sequence consists of the base sequence of SEQ ID NO:55, SEQ ID NO:56, or an RNA/DNA combination equivalent thereof.

18. The set of capture probes of claim 17 , wherein the base sequence of the first capture probe consists of the base sequence of SEQ ID NO:94 or SEQ ID NO:105.

19. The set of capture probes of claim 17 , wherein the base sequence of the second capture probe consists of the base sequence of SEQ ID NO:59.

20. The set of capture probes of claim 18 , wherein the base sequence of the second capture probe consists of the base sequence of SEQ ID NO:59.

Assignments (4)
SECURITY INTEREST Recorded Apr 8, 2026
From: BIOTHERANOSTICS, INC.; GEN-PROBE INCORPORATED; GEN-PROBE PRODESSE, INC.; CYTYC CORPORATION; SUROS SURGICAL SYSTEMS, INC.; GYNESONICS, INC.; BOLDER SURGICAL, LLC; FAXITRON BIOPTICS, LLC; HEALTH BEACONS, INC.; HOLOGIC, INC.
To: ROYAL BANK OF CANADA, AS COLLATERAL AGENT
Reel/Frame 075462/0440 →
SECURITY INTEREST Recorded Oct 14, 2019
From: HOLOGIC, INC.; CYNOSURE, LLC; CYTYC CORPORATION; FAXITRON BIOPTICS, LLC; FOCAL THERAPEUTICS, INC.; GEN-PROBE INCORPORATED
To: BANK OF AMERICA, N.A., AS COLLATERAL AGENT
Reel/Frame 050719/0701 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 30, 2019
From: DOCKTER, JANEL M.; WEINBAUM, BARBARA S.; SCHRODER, ASTRID R.W.
To: GEN-PROBE INCORPORATED
Reel/Frame 049324/0229 →
SECURITY INTEREST Recorded Jul 6, 2017
From: HOLOGIC, INC.; GEN-PROBE INCORPORATED; CYNOSURE, INC.
To: BANK OF AMERICA, N.A., AS COLLATERAL AGENT
Reel/Frame 042921/0111 →