Method and system for microbiome-derived diagnostics and therapeutics
A method for diagnosing and treating an immune microbial dysfunction in a subject, the method comprising: receiving an aggregate set of biological samples from a population of subjects; generating at least one of a microbiome composition dataset and a microbiome functional diversity dataset for the population of subjects; generating a characterization of the immune microbial dysfunction based upon features extracted from at least one of the microbiome composition dataset and the microbiome functional diversity dataset, wherein the characterization is diagnostic of at least one of Crohn's disease, inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), ulcerative colitis, and celiac disease; based upon the characterization, generating a therapy model configured to correct the immune microbial dysfunction; and at an output device associated with the subject, promoting a therapy to the subject based upon the characterization and the therapy model.
1. A method for diagnosing and treating an irritable bowel syndrome (IBS) condition of a subject, the method comprising:
receiving an aggregate set of samples from a population of subjects;
for each sample of the aggregate set of samples:
determining a microorganism nucleic acid sequence, comprising:
identifying a primer for a nucleic acid sequence associated with the IBS condition,
fragmenting nucleic acid material of the sample, and
amplifying the fragmented nucleic acid material with the identified primer; and
determining an alignment of the microorganism nucleic acid sequence to a reference nucleic acid sequence associated with the IBS condition;
generating a microbiome feature dataset for the population of subjects based upon the alignments;
generating a characterization of the IBS condition based upon a) a supplementary dataset informative of a characteristic associated with the IBS condition, and b) the microbiome feature dataset;
based upon the characterization, generating a therapy model that determines a therapy for correcting the IBS condition; and
at an output device associated with the subject, providing the therapy to the subject with the IBS condition based upon the characterization and the therapy model.
2. The method of claim 1 , wherein determining the microorganism nucleic acid sequence comprises performing, at a library preparation subsystem, multiplex amplification with the fragmented nucleic acid material based on the identified primer.
3. The method of claim 1 , wherein the primer for the nucleic acid sequence associated with the IBS condition comprises at least one unique identification sequence.
4. The method of claim 1 , wherein the microbiome feature dataset includes a microbiome taxonomic feature portion and a microbiome functional feature portion.
5. The method of claim 1 , further comprising: collecting the supplementary dataset, associated with at least a subset of the population of subjects, wherein the supplementary dataset is informative of health characteristics associated with the IBS condition.
6. The method of claim 5 , wherein generating the characterization further comprises performing a colonoscopy on the subject.
7. The method of claim 1 , wherein generating the characterization comprises performing a statistical analysis on the microbiome feature dataset, wherein features of the microbiome feature dataset comprises varying degrees of representation in a first subset of the population of subjects exhibiting the IBS condition and a second subset of the population of subjects not exhibiting the IBS condition.
8. The method of claim 1 , wherein generating the characterization of the IBS condition comprises evaluating features of the microbiome feature dataset associated with a set of taxonomic features comprising at least one of: Flavonifractor (genus), Odoribacter (genus), Blautia (genus), and Finegoldia (genus).
9. The method of claim 8 , wherein generating the characterization of the IBS condition comprises evaluating features of the microbiome feature dataset associated with the set of taxonomic features further comprising at least one of: Flavonifractor plautii (species), Holdemania (genus), Bacteroides (genus), Bacteroidaceae (family), Alistipes (genus), Rikenellaceae (family), bacterium NLAE-zl-P827 (species), Deltaproteobacteria (class), Bilophila (genus), Pasteurellaceae (family), Pasteurellales (order), Gammaproteobacteria (class), Bilophila wadsworthia (species).
10. The method of claim 1 , wherein generating the characterization of the IBS condition comprises evaluating features of the microbiome feature dataset associated with a set of functional features comprising a pcoC KEGG derived feature having a K07156 KEGG code.
11. The method of claim 10 , wherein generating the characterization of the IBS condition comprises evaluating features of the microbiome feature dataset associated with the set of functional features further comprising at least one of: a carboxylate-amine ligase feature and an isocitrate lyase feature.
12. A method for diagnosing and treating an irritable bowel syndrome (IBS) condition of a subject, the method comprising:
receiving a sample from the subject;
determining nucleic acid sequences of a microorganism component of the sample, comprising: identifying a primer for a nucleic acid sequence associated with the IBS condition, fragmenting nucleic acid material of the sample, and amplifying the fragmented nucleic acid material with the identified primer;
determining alignments of the nucleic acid sequence to reference nucleic acid sequences associated with the IBS condition;
generating a microbiome feature dataset for the subject based upon the alignments;
generating a characterization of the IBS condition in the subject upon processing the microbiome feature dataset with a characterization model derived from a population of subjects; and
at an output device associated with the subject, providing a therapy to the subject with the IBS condition upon processing the characterization with a therapy model designed to improve the IBS condition.
13. The method of claim 12 , wherein receiving the sample from the subject comprises providing a sampling kit to the subject at a remote location, the sampling kit including a sample container having a pre-process reagent component and configured to receive a sample from a collection site of the subject.
14. The method of claim 12 , wherein generating the characterization comprises evaluating features of the microbiome feature dataset associated with at least one of Flavonifractor (genus) and a pcoC feature.
15. The method of claim 14 , wherein generating the characterization comprises evaluating features of the microbiome feature dataset further associated with at least one of Odoribacter (genus), Blautia (genus), Finegoldia (genus), carboxylate-amine ligase and isocitrate lyase.
16. The method of claim 12 , wherein the microbiome feature dataset comprises transformation microbiome features derived from at least one of: relative abundance monotonic transformations and non-monotonic transformations.
17. The method of claim 12 , wherein providing the therapy comprises providing a consumable to the subject, the consumable affecting a microorganism component that selectively supports modulation of microbiome function in the subject, associated with correction of the IBS condition, based on the therapy model.
18. The method of claim 17 , wherein providing the consumable comprises providing at least one of a prebiotic and a small-molecule based therapy to the subject.
19. The method of claim 12 , wherein providing the therapy comprises providing instructions to the subject regarding setup of a user account within a social networking system configured to provide microbiome-derived insights to the subject, and transmitting diagnostic information and behavioral therapeutic techniques, associated with correcting the IBS condition, through the social networking system.
20. The method of claim 12 , wherein the output device comprises a wearable treatment device.