Methods and apparatus for extracting doxorubicin from blood and measuring doxorubicin in blood
Doxorubicin is extracted from blood using anionic material, such as a resin comprising sulfonated polystyrene divinylbenzene beads, and polyethersulfone membrane, or both. After exposing the resin and/or membrane to blood in order to remove doxorubicin therefrom, the doxorubicin maybe extracted from the resin and/or membrane by exposing the material to an extraction solution, sonicating the extraction solution to enhance release of the doxorubicin, and repeating the exposure and sonication in order to remove substantially all of doxorubicin from the resin.
1. A method for measuring doxorubicin present in a patient's blood, said method comprising:
mixing a sample of the patient's blood with a silver nitrate solution;
adding a volume of methanol to the mixture;
further mixing the blood, silver nitrate, and methanol together for at least 10 seconds;
letting the mixed blood, silver nitrate, and methanol stand for at least 30 minutes to precipitate particles and form a supernatant;
separating the supernatant via chromatography, wherein the separated supernatant contains doxorubicin originally present in the blood sample; and
analyzing the separated supernatant via spectroscopy, wherein the UV absorbance of doxorubicin is measured.
2. The method of claim 1 , including the step of analyzing the separated supernatant via spectroscopy at 254 nm.
3. The method of claim 1 , including the step of analyzing the separated supernatant via spectroscopy at 480 nm.
4. The method of claim 1 , wherein a chromatography buffer comprises acetonitrile and NaH 2 PO 4 .
5. The method of claim 1 , wherein the total mixed volume of the sample of the patient's blood with the silver nitrate solution is below 200 μl.
6. The method of claim 1 , wherein the volume of methanol added to the mixture is less than or equal to 1 ml.