WILD-CARD-MODIFICATION SEARCH TECHNIQUE FOR PEPTIDE IDENTIFICATION
Embodiments of a computer system, a method, and a computer-program product (e.g., software) for analyzing tandem-mass-spectrometry data are described. Using this analysis technique, unanticipated chemical modifications to peptides associated with proteins can be identified. In particular, a modification called a wild-card modification is used to identify the most likely chemical modifications in the peptides. A wild-card modification allows the addition of any mass, typically any integer atomic mass within a range, to any one amino acid residue within a candidate peptide.
1 . A method for identifying potential modifications to peptides associated with a precursor peptide, the method comprising:
receiving tandem-mass-spectrometry data associated with the precursor peptide, wherein the data includes a measured mass value for the precursor peptide and a number of peak locations;
generating one or more theoretical spectra for a candidate peptide using known spectral information associated with the candidate peptide, which comprises:
determining at least one variable mass modification to at least one amino acid within the candidate peptide; and
varying the variable mass modification within a predetermined range; and
identifying the potential modifications to the candidate peptide by comparing peak locations of the received tandem-mass-spectrometry data with peak locations of the generated theoretical spectra.
2 . The method of claim 1 , wherein the identified potential modifications include a post-translational modification.
3 . The method of claim 1 , wherein the identified potential modifications include a substitution of an amino acid.
4 . The method of claim 3 , further comprising:
obtaining the known spectral information associated with the candidate peptide from a predetermined peptide database;
modifying the candidate peptide based on the substitution of the amino acid; and
adding the modified candidate peptide to the predetermined database.
5 . The method of claim 1 , wherein generating the theoretical spectra further comprises applying one of more known mass modifications to the candidate peptide.
6 . The method of claim 5 , wherein the known modifications include: methylation, dimethylation, oxidation, deamidation, carbamylation, phosphorylation or acetylation.
7 . The method of claim 5 , wherein the known modifications include a known amino acid substitution.
8 . The method of claim 1 , wherein generating a respective theoretical spectrum further comprising shifting peak locations of fragments containing the at least one amino acid based on the variable mass modification.
9 . The method of claim 8 , wherein identifying the potential modifications to the candidate peptide involves an iterative process, in which a variable mass modification identified in a given iteration is treated as a known modification in a subsequent iteration.
10 . The method of claim 8 , wherein identifying the potential modifications to the candidate peptide involves an iterative process, in which a variable mass modification identified in a given iteration is treated as a known amino acid substitution in a subsequent iteration.
11 . A non-transitory computer-readable storage medium storing instructions that when executed by a computer system cause the computer to perform a method for identifying potential modifications to peptides associated with a precursor peptide, the method comprising:
receiving tandem-mass-spectrometry data associated with the precursor peptide, wherein the data includes a measured mass value for the precursor peptide and a number of peak locations;
generating one or more theoretical spectra for a candidate peptide using known spectral information associated with the candidate peptide, which comprises:
determining at least one variable mass modification to at least one amino acid within the candidate peptide; and
varying the variable mass modification within a predetermined range; and
identifying the potential modifications to the candidate peptide by comparing peak locations of the received tandem-mass-spectrometry data with peak locations of the generated theoretical spectra.
12 . The storage medium of claim 11 , wherein the identified potential modifications include a post-translational modification.
13 . The storage medium of claim 11 , wherein the identified potential modifications include a substitution of an amino acid.
14 . The storage medium of claim 11 , wherein generating the theoretical spectra further comprises applying one of more known mass modifications to the candidate peptide.
15 . The storage medium of claim 14 , wherein the known modifications include: methylation, dimethylation, oxidation, deamidation, carbamylation, phosphorylation or acetylation.
16 . The storage medium of claim 14 , wherein the known modifications include a known amino acid substitution.
17 . The storage medium of claim 11 , wherein generating a respective theoretical spectrum further comprising shifting peak locations of fragments containing the at least one amino acid based on the variable mass modification.
18 . The storage medium of claim 17 , wherein identifying the potential modifications to the candidate peptide involves an iterative process, in which a variable mass modification identified in a given iteration is treated as a known modification in a subsequent iteration.
19 . The method of claim 17 , wherein identifying the potential modifications to the candidate peptide involves an iterative process, in which a variable mass modification identified in a given iteration is treated as a known amino acid substitution in a subsequent iteration.
20 . A computer system, comprising:
a processor;
a storage device storing instructions that when executed by the processor cause the processor to perform a method for identifying potential modifications to peptides associated with a precursor peptide, the method comprising:
receiving tandem-mass-spectrometry data associated with the precursor peptide, wherein the data includes a measured mass value for the precursor peptide and a number of peak locations;
generating one or more modified theoretical spectra for a candidate peptide using known spectral information associated with the candidate peptide, which comprises:
identifying one or more known mass modifications for the candidate peptide;
determining at least one variable mass modification to at least one amino acid within the candidate peptide;
varying the variable mass modification within a predetermined range to produce a set of varied mass modifications; and
for each varied mass modification, generating a modified theoretical spectrum for the candidate peptide by combining the known mass modifications and the variable mass modification; and
identifying the potential modifications to the candidate peptide by comparing peak locations of the received tandem-mass-spectrometry data with peak locations of each modified theoretical spectrum.