Virion-derived nanospheres for selective delivery of therapeutic and diagnostic agents to cancer cells
View Patent ↗The invention relates to methods for producing papilloma-derived nanosphere particles that contain therapeutic, diagnostic, or other agents. The invention also provides nanosphere particle preparations that are useful for selectively delivering therapeutic, diagnostic, and/or other agents to cancer cells of subjects without eliciting a serotype-specific immunogenic response in the subjects.
1. A nanosphere particle comprising a papillomavirus L1 capsid protein encoded by the nucleic acid sequence of SEQ ID NO: 2.
2. The nanosphere particle of claim 1 , wherein the nanosphere particle is associated with an agent.
3. The nanosphere particle of claim 2 , wherein the agent is a therapeutic agent.
4. The nanosphere particle of claim 3 , wherein the therapeutic agent is an inorganic molecule, an organic molecule or a biologic active molecule.
5. The nanosphere particle of claim 3 , wherein the therapeutic agent comprises a small molecule, a protein, a peptide, an antibody, a toxin, a nucleic acid, a radioisotope, a radiolabeled molecule, a metal, an inducer of DNA methylation, a modulator of gene expression, an immune modulator, an enzyme inhibitor, a kinase inhibitor, an apoptosis inducer, a metabolism inhibitor, or any combination thereof.
6. The nanosphere particle of claim 3 , wherein the therapeutic agent is an anticancer agent.
7. The nanosphere particle of claim 3 , wherein the therapeutic agent is a toxic agent.
8. The nanosphere particle of claim 2 , wherein the agent is a detectable moiety.
9. The nanosphere particle of claim 8 , wherein the detectable moiety is fluorescent.
10. The nanosphere particle of claim 2 , wherein the agent is attached to a surface of the nanosphere particle.
11. The nanosphere particle of claim 1 , wherein the nanosphere particle is free of host cell nucleic acid and viral nucleic acid.
12. The nanosphere particle of claim 1 further comprising papillomavirus L2 capsid protein.
13. The nanosphere particle of claim 1 , wherein the modified papillomavirus L1 capsid protein has at least one amino acid that is PEGylated.
14. The nanosphere particle of claim 1 , wherein the nanosphere particle is assembled in vitro from a plurality of modified papillomavirus L1 capsid proteins encoded by the nucleic acid sequence of SEQ ID NO: 2.
15. The nanosphere particle of claim 10 , wherein the agent is an anticancer agent.
16. The nanosphere particle of claim 10 , wherein the agent is a toxic agent.
17. The nanosphere particle of claim 15 further comprising papillomavirus L2 capsid protein.
18. The nanosphere particle of claim 16 further comprising papillomavirus L2 capsid protein.
19. A papillomavirus L1 capsid protein encoded by the nucleic acid sequence of SEQ ID NO: 2.
20. A nucleic acid comprising the nucleic acid sequence of SEQ ID NO: 2.