Methods of treatment using purified (decolorized) aloe vera leaf dry juice
Disclosed herein is a method for improving the health of animal or human microflora using aloe vera . The method may induce the beneficial effect of activation of natural killer T cells. A method of purifying decolorized dried aloe vera leaf juice is also disclosed.
1. A method for inducing a beneficial effect on a human microbiome, the method comprising:
administering a composition to the gastrointestinal system of a human, the composition comprising decolorized aloe vera juice and one or more excipients,
wherein the aloe vera comprises an effective amount to result in a concentration from about 0.507 g/L to about 1.014 g/L of the composition upon administration to the gastrointestinal system of the human, and
wherein the beneficial effect is selected from the group consisting of an increased production of short chain fatty acids in the proximal colon, an increased total microbial population in the proximal colon, an increased production of acetic acid in the proximal colon, an increased production of propionic acid in the proximal colon, an increased population of the total bacteria in the proximal colon, an increased population of the transient concentration of bifidobacteria in the proximal colon, antioxidant protection within cells exposed to free radicals, activation of natural killer cells, activation of natural killer T cells, an increase in the production of Th2-type cytokines, a decrease in IFN-γ, promotion of M1 macrophages into M2 macrophages, and repair of injury to a monolayer of Caco-2 cells.
2. The method of claim 1 , wherein the beneficial effect on the human microbiome is an increased production of short chain fatty acids in the proximal colon.
3. The method of claim 1 , wherein the beneficial effect on the human microbiome is repair of injury to a monolayer of Caco-2 cells.
4. The method of claim 1 , wherein the beneficial effect on the human microbiome is antioxidant protection within cells exposed to free radicals.
5. The method of claim 1 , wherein the beneficial effect on the human microbiome is selected from the group consisting of activation of natural killer cells, activation of natural killer T cells, and an increase in the production of Th2-type cytokines.
6. The method of claim 5 , wherein the Th2-type cytokines are IL-10 or IL-5.
7. The method of claim 1 , wherein the beneficial effect on the human microbiome is a decrease in IFN-γor promotion of M1 macrophages into M2 macrophages.
8. The method of claim 1 , wherein the beneficial effect on the human microbiome is an increased total microbial population in the proximal colon.
9. The method of claim 1 , wherein the beneficial effect on the human microbiome is an increased production of acetic acid in the proximal colon.
10. The method of claim 1 , wherein the beneficial effect on the human microbiome is an increased production of propionic acid in the proximal colon.
11. The method of claim 1 , wherein the beneficial effect on the human microbiome is an increased production of total bacteria in the proximal colon.
12. The method of claim 1 , wherein the beneficial effect on the human microbiome is an increased production of the transient concentration of bifidobacteria in the proximal colon.
13. The method of claim 3 , wherein the injury to a monolayer of Caco-2 cells is injury caused by THP1 cells.
14. The method of claim 1 , wherein the composition further comprises an acidity modifier selected from the group consisting of citric acid salt, malic acid salt, acetic acid, acetic acid salt, lactic acid, lactic acid salt, tartaric acid, tartaric acid salt, formic acid, formic acid salt, propionic acid, propionic acid salt, butyric acid, butyric acid salt, valeric acid, valeric acid salt, phosphoric acid, and phosphoric acid salt.
15. The method of claim 1 , wherein the one or more excipients are selected from the group consisting of sorbic acid, sorbic acid salt, benzoic acid, benzoic acid salt, lactic acid, lactic acid salt, citric acid salt, malic acid salt, acetic acid, acetic acid salt, tartaric acid, tartaric acid salt, rosemary extract, lovage extract, chitosan, sage essential oil, thymol oil nisin, e-polylysine, grape seed extract, and goji berry extract.
16. The method of claim 1 , wherein the one or more excipients are selected from the group consisting of honey, fructose, dextrose, and maltodextrin.
17. The method of claim 1 , wherein the one or more excipients are selected from the group consisting of cellulose powder, modified starch, microcrystalline cellulose, magnesium stearate, stearic acid, sodium croscarmellose, calcium carbonate, and dicalcium phosphate.
18. The method of claim 1 , wherein the aloe vera juice comprises not more than about 10 ppm aloin.
19. The method of claim 1 , wherein the aloe vera juice comprises at least about 5% acetylated acemannan.