Chimpanzee clade E adenovirus nucleic acid-and amino acid-sequences, vectors containing same, and uses thereof
The present invention relates to novel adenovirus strains with an improved seroprevalence. In one aspect, the present invention relates to isolated polypeptides of adenoviral capsid proteins such as hexon, penton and fiber protein and fragments thereof and polynucleotides encoding the same. Also provided is a vector comprising the isolated polynucleotide according to the invention and adenoviruses comprising the isolated polynucleotides or polypeptides according to the invention and a pharmaceutical composition comprising said vector, adenovirus, polypeptide and/or polynucleotide. The invention also relates to the use of the isolated polypeptides, the isolated polypeptides, the vector, the adenoviruses and/or the pharmaceutical composition for the therapy or prophylaxis of a disease.
1. A recombinant adenoviral vector encoding a heterologous hexon protein comprising a polynucleotide that encodes an adenoviral hexon protein, wherein the polynucleotide is selected from the group consisting of:
(a) a polynucleotide encoding a polypeptide having the amino acid sequence of SEQ ID NO: 16;
(b) a polynucleotide encoding a polypeptide having the amino acid sequence of SEQ ID NO: 16, wherein the polypeptide comprises a deletion, insertion or substitution of not more than 35 amino acid residues; and
(c) a polynucleotide encoding a polypeptide having an amino acid sequence which is at least 90% identical over its entire length to the amino acid sequence of SEQ ID NO: 16;
further comprising at least one of a polynucleotide encoding a polypeptide having the amino acid sequence of SEQ ID NO: 22 and a polynucleotide encoding a polypeptide having the amino acid sequence of SEQ ID NO: 28.
2. The recombinant adenoviral vector of claim 1 wherein the vector comprises a polynucleotide encoding a polypeptide having the amino acid sequence of SEQ ID NO: 16, polynucleotide encoding a polypeptide having the amino acid sequence of SEQ ID NO: 22 and a polynucleotide encoding a polypeptide having the amino acid sequence of SEQ ID NO: 28.
3. The recombinant adenoviral vector of claim 2 , wherein the E4 region of the vector comprises ORF6 from human adenovirus.