Complementary color flashing for multichannel image presentation
Methods are provided for the highlighting of features in composite images through the alternating of images having complementary colors. An image having a feature of interest is used to generate one or more pseudo color images. A series of a pseudo color images and one or more additional pseudo color or original color images are then alternately displayed so that the differently colored regions among the series of images are easily recognizable to an operator. The differently colored regions differ in having hues that are complementary to one another. The methods are particularly useful for the display of information using two or more imaging modalities and channels, such as is the case for some medical applications in which a natural-light image of pink or light-red tissue with deeper red or blue vasculature is overlaid with another functional image. In these cases, a feature present in the functional image can be more easily perceived when displayed in a composite overlay with an underlying image from another imaging modality or channel.
1. A method of displaying images, the method comprising:
receiving a first image, wherein the first image comprises an original color and wherein the first image represents a view from a viewpoint;
acquiring a second image, wherein the second image represents a view from the viewpoint;
generating, using a computer, a third image by replacing the original color of the first image with a first false color having a first hue;
producing, using the computer, a fourth image by replacing the original color of the first image with a second false color having a second hue that is complementary to the first hue;
rendering a fifth image by overlaying the third image and the second image;
constructing a sixth image by overlaying the fourth image and the second image; and
alternately displaying the fifth and sixth images.
2. The method of claim 1 , wherein the first image has a minimum first image color value and a maximum first image color value, and wherein the first false color and the second false color have a false color value that is within the range between the minimum and maximum first image color values.
3. The method of claim 1 , wherein the alternately displaying is performed at a frequency.
4. The method of claim 3 , wherein the frequency of the alternately displaying is between 0.1 Hz and 25 Hz.
5. The method of claim 3 , further comprising:
accepting operator input selecting the frequency of the alternately displaying.
6. The method of claim 1 , wherein the first and second images each represent views of a biological sample.
7. The method of claim 1 , wherein the first image is a fluorescence image, an X-ray image, a positron emission tomography (PET) image, a photon emission computed tomography (SPECT) image, a magnetic resonance imaging (MRI) image, a nuclear magnetic resonance (NMR) image, an ultrasound image, an autoradiography image, an immunohistochemistry image, or a microscopy image.
8. The method of claim 1 , wherein the second image is a reflected light image or a tomography image.
9. A method of displaying images, the method comprising:
receiving a first image, wherein the first image comprises an original color and wherein the first image represents a view from a viewpoint;
generating, using a computer, a second image by replacing the original color of the first image with a first false color having a first hue;
producing, using the computer, a third image by replacing the original color of the first image with a second false color having a second hue that is complementary to the first hue; and
alternately displaying the second and third images.
10. The method of claim 9 , wherein the first image has a minimum first image color value and a maximum first image color value, and wherein the first false color and the second false color have a false color value that is within the range between the minimum and maximum first image color values.
11. The method of claim 9 , wherein the alternately displaying is performed at a frequency, and wherein the method further comprises:
accepting operator input selecting the frequency of the alternately displaying.
12. The method of claim 9 , wherein the first image is a reflected light image, a fluorescence image, an X-ray image, a positron emission tomography (PET) image, a photon emission computed tomography (SPECT) image, a magnetic resonance imaging (MRI) image, a nuclear magnetic resonance (NMR) image, an ultrasound image, an autoradiography image, an immunohistochemistry image, or a microscopy image.
13. The method of claim 9 , further comprising:
accepting operator input selecting the viewpoint.
14. A method of displaying images, the method comprising:
receiving a first image, wherein the first image comprises an original color having a first hue and wherein the first image represents a view from a viewpoint;
acquiring a second image, wherein the second image represents a view from the viewpoint;
generating, using a computer, a third image by replacing the original color of the first image with a false color having a second hue that is complementary to the first hue;
rendering a fourth image by overlaying the third image and the second image;
constructing a fifth image by overlaying the first image and the second image; and
alternately displaying the fourth and fifth images.
15. The method of claim 14 , wherein the first image has a minimum first image color value and a maximum first image color value, and wherein the false color has a false color value that is within the range between the minimum and maximum first image color values.
16. The method of claim 14 , wherein the alternately displaying is performed at a frequency.
17. The method of claim 16 , wherein the frequency of the alternately displaying is between 0.1 Hz and 25 Hz.
18. The method of claim 16 , further comprising:
accepting operator input selecting the frequency of the alternately displaying.
19. The method of claim 14 , wherein the first and second images each represent views of a biological sample.
20. The method of claim 14 , wherein the first image is a fluorescence image, an X-ray image, a positron emission tomography (PET) image, a photon emission computed tomography (SPECT) image, a magnetic resonance imaging (MRI) image, a nuclear magnetic resonance (NMR) image, an ultrasound image, an autoradiography image, an immunohistochemistry image, or a microscopy image.