Evaluating a pluripotent stem cell based on a region of a phase distribution image having a phase amount less than a threshold phase amount of a mitochondrion in a somatic cell
An operation device includes: a communication unit that receives signals of images of a biological sample and outputs data to a display medium, the images being captured by a microscope that converts a phase distribution into an image intensity distribution; and a calculator that calculates a first phase distribution of the biological sample from the image signals. The calculator extracts a region having a phase amount that is not less than a specified phase amount from the first phase distribution, and generates evaluation information by using the region having a phase amount that is not less than the specified phase amount, that is an indicator used when a user evaluates a state of the biological sample; and the communication unit outputs the evaluation information to the display medium.
1. A method for assisting in evaluating a pluripotent stem cell without stains, the method comprising:
forming a phase distribution image of the pluripotent stem cell from images of the pluripotent stem cell, the images of the pluripotent stem cell being captured using a microscope that converts a phase distribution into an image intensity distribution;
setting, as a threshold, a phase amount of a mitochondrion in a somatic cell;
extracting, from the phase distribution image, an image region having a phase amount that is not less than the threshold;
generating first information distinguishing the phase distribution image from the image region;
generating evaluation information indicating an extent to which the pluripotent stem cell remains pluripotent, the evaluation information being generated based on the first information; and
displaying the evaluation information on an image display medium.
2. The method according to claim 1 , wherein the evaluation information comprises an evaluation image in which the image region is superimposed over the phase distribution image.
3. The method according to claim 1 , wherein the first information is an area of the image region.
4. The method according to claim 1 , wherein a differential interference contrast microscope is used as the microscope.
5. The method according to claim 1 , wherein a phase contrast microscope is used as the microscope.
6. The method according to claim 1 , wherein an oblique illumination microscope is used as the microscope.
7. The method according to claim 1 , wherein a decentered aperture microscope is used as the microscope.
8. The method according to claim 1 , wherein the images of the pluripotent stem cell include, for each shear direction, two phase distribution images of different image contrasts obtained under different polarization states such that a retardation thereof is ±θ.
9. The method according to claim 1 , wherein the generating the first information comprises generating an image in which the image region is superimposed over the phase distribution image.
10. The method according to claim 1 , wherein the displaying the evaluation information on the image display medium includes superimposing, with a specified color, the image region over the phase distribution image and displaying a resultant image on the image display medium.
11. The method according to claim 1 , further comprising:
calculating an area within the phase distribution image occupied by the image region.
12. The method according to claim 11 , further comprising:
calculating a proportion of the area occupied by the image region within the phase distribution image.
13. The method according to claim 1 , wherein the evaluation information is generated by calculating a proportion of an area of the pluripotent stem cell occupied by the image region.
14. A device comprising:
a communication unit and
a computer configured to:
form a phase distribution image of a pluripotent stem cell from images of the pluripotent stem cell, the images of the pluripotent stem cell being captured using a microscope that converts a phase distribution into an image intensity distribution and being received from the microscope via the communication unit;
set, as a threshold, a phase amount of a mitochondrion in a somatic cell;
extract, from the phase distribution image, an image region having a phase amount that is not less than the threshold;
generate first information distinguishing the phase distribution image from the image region;
generate evaluation information indicating an extent to which the pluripotent stem cell remains pluripotent, the evaluation information being generated based on the first information; and
output, via the communication unit, the evaluation information to an image display medium so as to display the evaluation information on the image display medium.
15. A microscope system comprising:
a microscope configured to convert a phase distribution into an image intensity distribution;
a display; and
a computer communicably connected with the microscope and the display, the computer being configured to:
form a phase distribution image of a pluripotent stem cell from images of the pluripotent stem cell, the images of the pluripotent stem cells being captured using the microscope and received from the microscope;
set, as a threshold, a phase amount of a mitochondrion in a somatic cell;
extract, from the phase distribution image, an image region having a phase amount that is not less than the threshold;
generate first information distinguishing the phase distribution image from the image region;
generate evaluation information indicating an extent to which the pluripotent stem cell remains pluripotent, the evaluation information being generated based on the first information; and
output the evaluation information to the display so as to display the evaluation information on the display.