ADDITIVE CHANNELS
Compositions, devices and methods are described for preventing, reducing, controlling or delaying adhesion, adsorption, surface-mediated clot formation, or coagulation in a microfluidic device or chip. In one embodiment, blood (or other fluid with blood components) that contains anticoagulant is introduced into a microfluidic device comprising one or more additive channels containing one or more reagents that will re-activate the native coagulation cascade in the blood that makes contact with it “on-chip” before moving into the experimental region of the chip.
1 . A microfluidic device comprising i) a microchannel in fluidic communication with ii) an input port and iii) an output port, iv) one or more first additive channels in fluidic communication with at least one microchannel, positioned at or near said input port.
2 . The microfluidic device of claim 1 , wherein one first additive channel is positioned on one side of said microchannel near said input port.
3 . The microfluidic device of claim 2 , wherein another first additive channel is positioned on another side of said microchannel near said input port.
4 . The microfluidic device of claim 1 , wherein said microchannel comprises an active region comprising cells.
5 . The microfluidic device of claim 1 , further comprising v) one or more second additive channels in fluidic communication with said microchannel, positioned at or near said output port.
6 . The microfluidic device of claim 5 , wherein one second additive channel is positioned on one side of said microchannel near said output port.
7 . The microfluidic device of claim 5 , wherein another second additive channel is positioned on another side of said microchannel near said output port.
8 . A system, comprising:
a) a fluid sample comprising anticoagulant, said fluid sample disposed in
b) a microfluidic device comprising i) a microchannel in fluidic communication with ii) an input port and iii) an output port, iv) one or more first additive channels in fluidic communication with at least one microchannel, positioned at or near said input port, said first additive channels comprising a first reagent solution comprising one or more reagents capable of re-activating the coagulation cascade.
9 . The system of claim 8 , wherein said microfluidic device comprises v) one or more second additive channels in fluidic communication with said microchannel, positioned at or near said output port.