Maize event DP-004114-3 and methods for detection thereof
The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-004114-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
1. A pair of polynucleotide primers comprising a first polynucleotide primer and a second polynucleotide primer, wherein the first polynucleotide primer comprises the nucleotide sequence selected from SEQ ID NO: 29 and the complement thereof; and the second polynucleotide primer comprises the nucleotide sequence selected from SEQ ID NO: 30 and the complement thereof, which function together in the presence of a DP-004114-3 DNA template in a sample to produce an amplicon diagnostic for event DP-004114-3, wherein a detectable label or reporter molecule is attached to the first and/or second polynucleotide primer.
2. A kit for detecting nucleic acids that are unique to event DP-004114-3 comprising a primer pair selected from the group consisting of SEQ ID NOs: 29 and 30, SEQ ID NOs: 33 and 34, and the complements thereof, wherein a detectable label or reporter molecule is attached to the first and/or second polynucleotide primer, and which upon amplification of or hybridization to a target nucleic acid sequence in a sample followed by detection of the amplicon or hybridization to the target sequence, are diagnostic for the presence of nucleic acid sequences unique to event DP-004114-3 in the sample.