Compositions and methods of cell attachment
Compositions, devices and methods are described for improving adhesion, attachment, and/or differentiation of cells in a microfluidic device or chip. In one embodiment, one or more ECM proteins are covalently coupled to the surface of a microchannel of a microfluidic device. The microfluidic devices can be stored or used immediately for culture and/or support of living cells such as mammalian cells, and/or for simulating a function of a tissue, e.g., a liver tissue, muscle tissue, etc. Extended adhesion and viability with sustained function over time is observed.
1. A microfluidic device for culturing cells, comprising;
a) a plasma treated polydimethylsiloxane membrane surface, said surface disposed within a microchannel;
b) an N-sulphosuccinimidyl-6-(4′-azido-2′-nitrophenylamino) hexanoate crosslinker reacted with at least one portion of said surface with an ultraviolet light reactive moiety of said crosslinker;
c) one or more extracellular matrix proteins or peptides covalently attached to a chemically reactive moiety of said N-sulphosuccinimidyl-6-(4′-azido-2′-nitrophenylamino) hexanoate crosslinker;
d) a fluidic source in fluidic communication with said microchannel, wherein said fluidic source is configured to create flow conditions; and
e) a plurality of epithelial cells that remain viable and attached to said extracellular matrix proteins or peptides for at least 7 days in said flow conditions.
2. The device of claim 1 , wherein said surface comprises a linear pattern.
3. The device of claim 1 , wherein said plurality of epithelial cells are hepatocytes.
4. The device of claim 1 , wherein said membrane surface is a porous membrane surface.
5. A microfluidic device for culturing cells, comprising;
a) a plasma treated polydimethylsiloxane membrane surface disposed within a microchannel, wherein at least a portion of said surface has reacted with an ultraviolet light-reactive moiety of a N-sulphosuccinimidyl-6-(4′-azido-2′-nitrophenylamino) hexanoate crosslinker;
b) a fluidic source in fluidic communication with said microchannel, wherein said fluidic source is configured to create a fluid flow;
c) one or more extracellular matrix proteins or peptides covalently attached to a chemically reactive moiety of said N-sulphosuccinimidyl-6-(4′-azido-2′-nitrophenylamino) hexanoate crosslinker; and
d) a plurality of cultured epithelial cells that remain attached to said one or more extracellular matrix proteins or peptides under said fluid flow for at least 7 days.
6. The device of claim 5 , wherein said surface comprises a linear pattern.
7. The device of claim 5 , wherein said membrane surface is a porous membrane surface.