Method for target substance detection and measurement
Various embodiments for a hand-held device to determine a level of one or more cannabinoid compounds in exhaled human breath are provided; some embodiments may also determine a level of alcohol in the exhaled breath. The device may perform any of the following steps: capture, concentrate, separate, identify, and quantify the level of cannabinoids in the exhaled human breath. The device may use solvent extraction and liquid chromatography to concentrate and separate cannabinoids from breath contaminants, followed by selective chemical modification of the cannabinoids with a fluorescent indicator for quantification. The measurement methodology may also include real time calibration of the device and detection protocol using known cannabinoid standards, which are simultaneously analyzed along with an unknown, to quantify the level of cannabinoids captured from the breath sample. The capture and separation components, along with cannabinoid analysis standards, may be configured as part of a single-use, field-replaceable testing cartridge.
1. A method for detecting a substance in human breath, comprising:
capturing a substance from a breath sample;
combining a diazotized fluorophore with the captured substance in a solution to form an adduct with the substance;
exposing the adduct to a light source to produce a luminescence;
measuring the luminescence; and
determining a quantity of the substance based on the measured luminescence.
2. The method of claim 1 , wherein the substance is tetrahydrocannabinol.
3. The method of claim 2 , wherein the diazotized fluorophore is a rhodamine.
4. The method of claim 3 , wherein the rhodamine is Rhodamine-123.
5. The method of claim 2 , wherein a second substance is captured and collected from the breath sample, and both tetrahydrocannabinol and the second substance are detected from a single breath sample.
6. The method of claim 5 , wherein the second substance is ethanol.