ISOLATION OF ADULT MULTIPOTENTIAL CELLS BY TISSUE NON-SPECIFIC ALKALINE PHOSPHATASE
The present invention relates to the use of tissue non-specific alkaline phosphatase (TNAP) as a marker for identifying and/or isolating adult multipotential cells. The present invention also relates to cell populations enriched by methods of the present invention and therapeutic uses of these cells.
1 - 45 . (canceled)
46 . An enriched population of TNAP + adult multipotential cells, wherein at least 4% of the total cell population are TNAP + , STRO-1 + multipotential cells capable of being cultured in vitro to produce adipocytes, osteocytes and chondrocytes, wherein the TNAP + marker is a TNAP + marker which binds the STRO-3 antibody produced by the hybridoma cell line deposited under ATCC Accession Number PTA-5582.
47 . An enriched population according to claim 46 , wherein at least 10% of the total cell population are TNAP + , STRO-1 + multipotential cells capable of being cultured in vitro to produce adipocytes, osteocytes and chondrocytes.
48 . An enriched population according to claim 46 , wherein at least 20% of the total cell population are TNAP + , STRO-1 + multipotential cells capable of being cultured in vitro to produce adipocytes, osteocytes and chondrocytes.
49 . An enriched population according to claim 46 , wherein the STRO-1 + cells are STRO-1 bright .
50 . An enriched population according to claim 47 , wherein the STRO-1 + cells are STRO-1 bright .
51 . An enriched population according to claim 48 , wherein the STRO-1 + cells are STRO-1 bright .
52 . A method of generating a committed cell population of a specific tissue type, the method comprising culturing a population of adult multipotential cells according to claim 46 in the presence of one or more stimulatory factors; and subjecting said cultured population to conditions biasing differentiation of the adult muitipotential cells to the specific tissue type.
53 . A method of generating a committed cell population of a specific tissue type, the method comprising culturing a population of adult multipotential cells according to claim 47 in the presence of one or more stimulatory factors; and subjecting said cultured population to conditions biasing differentiation of the adult muitipotential cells to the specific tissue type.
54 . A method of generating a committed cell population of a specific tissue type, the method comprising culturing a population of adult multipotential cells according to claim 48 in the presence of one or more stimulatory factors; and subjecting said cultured population to conditions biasing differentiation of the adult muitipotential cells to the specific tissue type.
55 . A method of generating a committed cell population of a specific tissue type, the method comprising culturing a population of adult multipotential cells according to claim 49 in the presence of one or more stimulatory factors; and subjecting said cultured population to conditions biasing differentiation of the adult muitipotential cells to the specific tissue type.
56 . A composition comprising the enriched population of adult multipotential cells according to claim 46 .
57 . A composition comprising the enriched population of adult multipotential cells according to claim 47 .
58 . A composition comprising the enriched population of adult multipotential cells according to claim 48 .
59 . A composition comprising the enriched population of adult multipotential cells according to claim 49 .
60 . A method for generating or repairing tissue in a subject in need thereof, the method comprising administering to the subject the enriched cell population according to claim 46 so as to generate or repair the tissue.
61 . A method for generating or repairing tissue in a subject in need thereof, the method comprising administering to the subject the enriched cell population according to claim 47 so as to generate or repair the tissue.
62 . A method for generating or repairing tissue in a subject in need thereof, the method comprising administering to the subject the enriched cell population according to claim 48 so as to generate or repair the tissue.
63 . A method for generating or repairing tissue in a subject in need thereof, the method comprising administering to the subject the enriched cell population according to claim 49 so as to generate or repair the tissue.
64 . A method for generating or repairing tissue in a subject in need thereof, the method comprising administering to the subject the composition according to claim 56 so as to generate or repair the tissue.