Antagonists of IL-6 to raise albumin and/or lower CRP
The present invention is directed to therapeutic methods using IL-6 antagonists such as antibodies and fragments thereof having binding specificity for IL-6 to improve survivability or quality of life of a patient in need thereof. In preferred embodiments, the anti-IL-6 antibodies will be humanized and/or will be aglycosylated. Also, in preferred embodiments these patients will comprise those exhibiting (or at risk of developing) an elevated serum C-reactive protein level or a reduced serum albumin level prior to treatment. In another preferred embodiment, the patient's Glasgow Prognostic Score will be increased and survivability will preferably be improved.
1. A method of reducing a patient's serum C-reactive protein (“CRP”) level and/or increasing human serum albumin (“HSA”) level in a patient that has a disease associated with chronic inflammation comprising administering a therapeutically effective amount of an antibody or antibody fragment comprising a light chain comprising CDR polypeptides having the amino acid sequences of SEQ ID NO: 4, 5 and 6; and a heavy chain comprising CDR polypeptides having the amino acid sequences of SEQ ID NO: 7, 8 or 120 and 9.
2. The method of claim 1 wherein the patient has elevated CRP levels prior to treatment.
3. The method of claim 1 wherein the patient has reduced HSA levels prior to treatment.
4. The method of claim 1 wherein the patient has elevated CRP levels and reduced HSA levels prior to treatment.
5. The method of claim 1 wherein the patient has a chronic inflammatory condition, autoimmune disease, cancer or a viral condition.
6. The method of claim 1 , wherein the antibody or antibody fragment comprises a light chain comprising CDR polypeptides having SEQ ID NO:4, 5 and 6; and a heavy chain comprising CDR polypeptides having the amino acid sequences of SEQ ID NO:7, 120 and 9.
7. The method of claim 1 , wherein the antibody or antibody fragment comprises a light chain comprising CDR polypeptides having the amino acid sequences of SEQ ID NO:4, 5 and 6; and a heavy chain comprising CDR polypeptides having the amino acid sequences of SEQ ID NO:7, 8 and 9.
8. The method of claim 1 , wherein the antibody or antibody fragment comprises a variable light chain polypeptide having an amino acid sequence possessing at least 90% sequence identity to SEQ ID NO:20; and a variable heavy chain polypeptide having an amino acid sequence possessing at least 90% sequence identity to SEQ ID NO:18 or 19.
9. The method of claim 1 , wherein the antibody or antibody fragment comprises a variable light chain polypeptide having an amino acid sequence possessing at least 95% sequence identity to SEQ ID NO:20; and a variable heavy chain polypeptide having an amino acid sequence possessing at least 95% sequence identity to SEQ ID NO:18 or 19.
10. The method of claim 1 , wherein the antibody or antibody fragment comprises a variable light chain polypeptide having an amino acid sequence possessing at least 98% sequence identity to SEQ ID NO:20; and a variable heavy chain polypeptide having an amino acid sequence possessing at least 98% sequence identity to SEQ ID NO:18 or 19.
11. The method of claim 1 , wherein the antibody or antibody fragment comprises a variable light chain polypeptide having an amino acid sequence possessing at least 99% sequence identity to SEQ ID NO:20; and a variable heavy chain polypeptide having an amino acid sequence possessing at least 99% sequence identity to SEQ ID NO:18 or 19.
12. The method of claim 1 , wherein the antibody or antibody fragment comprises a variable light chain polypeptide comprising the amino acid sequence of SEQ ID NO: 20; and a variable heavy chain polypeptide comprising the amino acid sequence of SEQ ID NO: 18 or 19.
13. The method of claim 1 , wherein the antibody or antibody fragment comprises a variable light chain polypeptide comprising the amino acid sequence of SEQ ID NO: 20; and a variable heavy chain polypeptide comprising the amino acid sequence of SEQ ID NO: 19.
14. The method of claim 13 , wherein the antibody or antibody fragment comprises IgG1 constant region polypeptides.
15. The method of claim 14 , wherein the IgG1 constant region polypeptides comprise the amino acid sequences of SEQ ID NO: 586 and 588.
16. The method of claim 1 , wherein the antibody or antibody fragment comprises a variable light chain polypeptide comprising the amino acid sequence of SEQ ID NO: 20; and a variable heavy chain polypeptide comprising the amino acid sequence of SEQ ID NO: 18.
17. The method of claim 1 , wherein the antibody or antibody fragment comprises IgG1, IgG2, IgG3 or IgG4 constant region polypeptides.
18. The method of claim 1 , wherein the antibody or antibody fragment comprises IgG1 constant region polypeptides.
19. The method of claim 18 , wherein the IgG1 constant region polypeptides comprise the amino acid sequences of SEQ ID NO: 586 and 588.