Cell analysis apparatus and method
A cell analysis apparatus including: a cell image acquisition unit that acquires an image of cells in a culture vessel in which cells are cultured; a smoothing processing unit that smooths luminance values in the image acquired by the cell image acquisition unit; a minimum-value detecting unit that detects minimum values of the luminance values smoothed by the smoothing processing unit; a smallest-minimum-value extracting unit that extracts smallest minimum values which are the smallest in regions according to the sizes of the cells, from the minimum values detected by the minimum-value detecting unit; a counting unit that counts the number of smallest minimum values extracted by the smallest-minimum-value extracting unit; and a cell-count calculating unit that calculates the number of cells in the culture vessel on the basis of the number of smallest minimum values counted by the counting unit.
1. A cell analysis apparatus comprising a processor, the processor comprising hardware and configured to execute the steps of:
acquiring a first image of cells in a culture vessel in which cells are cultured;
creating a second image by smoothing luminance values in the first image;
detecting minimum values of the luminance values from the second image;
extracting smallest minimum values each of which is the smallest among the minimum values in a search region, wherein the search region corresponds to each of the minimum values and is defined according to the sizes of the cells;
counting a number of the smallest minimum values; and
calculating the number of cells in the culture vessel on the basis of the number of the smallest minimum values.
2. A cell analysis apparatus according to claim 1 , wherein the search region is defined as a continuous region containing said minimum value and having luminance values less than or equal to a value obtained by adding a prescribed threshold to said minimum value.
3. A cell analysis apparatus according to claim 2 , wherein the processor executes the step of extracting the smallest minimum values by repeatedly:
setting each of the minimum values a smallest minimum value candidate;
extracting, as the smallest minimum value, one of the smallest minimum value candidates for which the luminance value is the smallest;
setting, as a neighboring region, a region that is closer to the smallest minimum value than a region already set as a neighboring region, in the search region; and
excluding all of the minimum values existing in the neighboring region from the smallest minimum value candidates, until there are no more of the smallest minimum value candidates.
4. A cell analysis apparatus according to claim 2 , wherein the processor executes the step of extracting the smallest minimum values by repeatedly:
setting each of the minimum values as a smallest minimum value candidate;
extracting one for which the luminance value is the smallest in the smallest minimum value candidates as the smallest minimum value;
setting, to a neighboring region, a continuous region having luminance values larger than the smallest minimum value that is already set, in the search region; and
excluding all of the minimum values existing in the neighboring region from the smallest minimum value candidates, until there are no more of the smallest minimum value candidates.
5. A cell analysis apparatus according to claim 1 , wherein the processor is configured to further execute a step of:
extracting a cell region in which cells exist, in the first image, and
wherein the minimum values of the luminance values in the second image are detected in the cell region.
6. A cell analysis apparatus comprising a processor, the processor comprising hardware and configured to execute the steps of:
acquiring a first image of cells in a culture vessel in which cells are cultured;
creating a second image by smoothing luminance values in the first image;
detecting maximum values of the luminance values from the second image;
extracting largest maximum values each of which is the largest among the maximum values in a search region, wherein the search region corresponds to each of the maximum values and is defined according to the sizes of the cells;
counting a number of the largest maximum values; and
calculating the number of cells in the culture vessel on the basis of the number of the largest maximum values.
7. A cell analyzing method comprising:
an image-capturing step of acquiring a first image of cells in a culture vessel in which the cells are cultured;
a creating a second image step by smoothing luminance values in the first image;
a detecting step of detecting minimum values of the luminance values from the second image;
an extracting step of extracting smallest minimum values each of which is the smallest among the minimum values in a search region, wherein the search region corresponds to each of the minimum values and is defined according to the sizes of the cells;
a counting step of counting a number of the smallest values; and
a cell-count calculating step of calculating the number of cells in the culture vessel on the basis of the number of the smallest minimum values.
8. A cell analysis method according to claim 7 , wherein the search region is defined as a continuous region containing each of the minimum values and having luminance values less than or equal to a value obtained by adding a prescribed threshold to each of the minimum values.
9. A cell analysis method according to claim 8 , wherein the extracting step repeats:
a step of setting each of the minimum values a smallest minimum value candidate;
a step of extracting, as a smallest minimum value, one of the smallest minimum value candidates for which the luminance value is the smallest;
a step of setting, as a neighboring region, a region closer to the smallest minimum value than a region already set as a neighboring region, in the search region; and
a step of excluding all of the minimum values that exist in the neighboring region from the smallest minimum value candidates, until there are no more of the smallest minimum value candidates.
10. A cell analysis method according to claim 8 , wherein the extracting step repeats:
a step of setting each of the minimum values as a smallest minimum value candidate;
a step of extracting, as the smallest minimum value, one of the smallest minimum value candidates for which the luminance value is the smallest;
a step of setting, as a neighboring region, a continuous region having luminance values larger than the smallest minimum value that is already set, in the search region; and
a step of excluding all of the minimum values that exist in the neighboring region from the smallest minimum value candidates, until there are no more of the smallest minimum value candidates.
11. A cell analysis method according to claim 7 , further comprising:
a cell-region extracting step of extracting a region in which cells exist, in the first image, and
wherein the minimum values of the luminance values in the second image are detected in the cell region.
12. A cell analyzing method comprising:
an image-capturing step of acquiring a first image of cells in a culture vessel in which the cells are cultured;
a creating a second image step by smoothing luminance values in the first image;
a detecting step of detecting maximum values of the luminance values from the second;
an extracting step of extracting largest maximum values each of which is the largest among the maximum values in a search region, wherein the search region corresponds to the maximum values and is defined according to the sizes of the cells;
a counting step of counting a number of the largest maximum values; and
a cell-count calculating step of calculating the number of cells in the culture vessel on the basis of the number of the largest maximum values.