Compositions and methods for controlling fungal and bacterial diseases in plants
The present invention provides a fungicidal composition comprising a strain of Bacillus subtilis or Bacillus amyloliquefaciens and one of several compounds in a synergistically effective amount. Also provided are methods of controlling fungal harmful organisms and/or bacterial harmful organisms in a plant, the method comprising applying an effective amount of a fungicidal composition to the plant, to a part of the plant and/or to a locus on which the plant or plant part grows.
1. A fungicidal composition comprising:
a) a lipopeptide-producing strain of Bacillus subtilis QST713 or a fermentation broth of Bacillus subtilis QST713 that is substantially free of cells; and
b) a polyalkylene compound;
of formula (I):
wherein
m is an integer between 1 and 20;
n is an integer between 1 and 5;
Z 1 is CH 2 or O;
and R 1 is a sulfate or a phosphate;
or a geometrical isomer, optical isomer, enantiomer, diastereoisomer, tautomer, or an agriculturally acceptable salt thereof;
in a synergistically effective amount.
2. The fungicidal composition of claim 1 , wherein R 1 is a sulfate.
3. The fungicidal composition of claim 2 , wherein the polyalkylene compound is an alkyl sulfate of formula (I) or an agriculturally acceptable salt thereof.
4. The fungicidal composition of claim 3 , wherein the polyalkylene compound is lauryl sulfate, myristyl sulfate, palmityl sulfate, stearyl sulfate or an agriculturally acceptable salt thereof.
5. The fungicidal composition of claim 1 , wherein the polyalkylene compound is a lauryl ether sulfate having the formula:
wherein o is an integer between 1 and 5;
or an agriculturally acceptable salt thereof.
6. The fungicidal composition of claim 1 , wherein the weight to weight ratio of the lipopeptide-producing strain of Bacillus subtilis or QST713 or the fermentation broth to the polyalkylene is about 500:1 to about 1:500.
7. The fungicidal composition of claim 1 , wherein the strain of Bacillus subtilis QST713 is part of a fermentation product.
8. A plant or plant part coated with the fungicidal composition according to claim 1 .
9. A fungicidal composition comprising:
a) a lipopeptide-producing strain of Bacillus subtilis QST713 or a fermentation broth of Bacillus subtilis QST713 that is substantially free of cells; and
b) a sulfonate or an agriculturally acceptable salt thereof;
wherein
the sulfonate is a compound of formula (IV)
wherein
R 1 is a linear or branched C 1-20 alkyl or a linear or branched C 2-20 alkene; and
M is H + , Li + , Na + , K + or (C 1-8 alkyl) 4 N + ; or
the sulfonate is a compound of formula (V)
wherein
R 1 and R 2 are independently a linear or branched C 1-20 alkyl or a linear or branched C 2-20 alkene; and
M is H + , Li + , Na + , K + or (C 1-8 alkyl) 4 N + ;
in a synergistically effective amount.
10. The fungicidal composition of claim 9 , wherein the sulfonate is the compound of formula (IV):
wherein
R 1 is a linear or branched C 1-20 alkyl or a linear or branched C 2-20 alkene; and
M is H + , Li + , Na + , K + or (C 1-8 alkyl) 4 N + .
11. The fungicidal composition of claim 10 , wherein R 1 is a linear C 8-16 alkyl.
12. The fungicidal composition of claim 11 , wherein the sulfonate is tetradecene sulfonate, hexadecene sulfonate, or a Li + , Na + , K + or (C 1-8 alkyl) 4 N + salt thereof.
13. The fungicidal composition of claim 9 , wherein the sulfonate is the compound of formula (V):
wherein
R 1 and R 2 are independently a linear or branched C 1-20 alkyl or a linear or branched C 2-20 alkene; and
M is H + , Li + , Na + , K + or (C 1-8 alkyl) 4 N + .
14. The fungicidal composition of claim 13 , wherein R 1 and R 2 are independently linear or branched C 8 alkyl.
15. The fungicidal composition of claim 14 , wherein the sulfonate is dioctyl sulfosuccinate; 1,4-bis(2-ethylhexoxy)-1,4-dioxobutane-2-sulfonate; or a Li + , Na + , K + or (C 1-8 alkyl) 4 N + salt thereof.
16. The fungicidal composition of claim 9 , wherein the weight to weight ratio of the lipopeptide-producing strain of Bacillus subtilis QST713 or the fermentation broth to the sulfonate is about 500:1 to about 1:500.
17. The fungicidal composition of claim 9 , wherein the strain of Bacillus subtilis QST713 is part of a fermentation product.