Methods for producing liquid compositions comprising β-glucan
The present invention relates to methods of processing mixtures comprising β-glucans and polysaccharides.
1. A method of processing a mixture comprising at least two polysaccharides, and no more than 5% w/w of oil, and no more than 1% w/w of protein, wherein one of the polysaccharides is β-glucan, and wherein at least one polysaccharide is not β-glucan, wherein said method comprises:
subjecting said mixture to at least one enzymatic treatment, said enzymatic treatment degrading at least a portion of at least one polysaccharide which is not β-glucan; and
filtering the enzymatically treated mixture to obtain a mixture comprising beta-glucan.
2. The method of claim 1 , wherein said mixture comprises no more than 1% w/w of oil.
3. The method of claim 1 , wherein said filtering comprises filtering with a membrane filter.
4. The method of claim 1 , wherein prior to enzymatic treatment and filtration, the β-glucan is present in said mixture at an amount of about 1.5% w/w.
5. The method of claim 1 , wherein said method comprises at least two enzymatic treatments.
6. The method of claim 5 , wherein at least one of said enzymatic treatments comprises treatment with an enzyme which degrades β-glucan, wherein the enzyme which degrades β-glucan is added for a pre-determined period of time before quenching the enzymatic reaction.
7. The method of claim 1 , wherein said enzymatic treatment comprises treatment with amyloglucosidase.
8. The method of claim 1 , wherein during said enzymatic treatment, said mixture is subjected to a high sheer force.
9. The method of claim 8 , wherein said mixture is subjected to said high sheer force at a temperature of between 95° C. and 109° C.
10. The method of claim 1 , wherein, prior to said enzymatic treatment, said mixture is heated to 135° C.
11. The method of claim 9 , wherein said mixture has a pH which is reduced to between about 1.4 and 2.0.
12. The method of claim 9 , wherein said mixture is allowed to cool naturally prior to said enzymatic treatment.
13. The method of claim 1 , wherein at least one enzymatic treatment step comprises heating said mixture, and wherein said mixture is subsequently allowed to cool naturally.
14. The method of claim 1 , wherein following said at least one enzymatic treatment, and prior to said filtration, said mixture is allowed to stand for at least two weeks.
15. The method of claim 14 , wherein following said at least one enzymatic treatment, and prior to said filtration, said mixture is allowed to stand for at least five weeks.
16. The method of claim 1 , wherein at least one polysaccharide which is not β-glucan is degraded to 6 DP.
17. The method of claim 1 , wherein at least one polysaccharide which is not β-glucan is degraded to 4 DP.
18. The method of claim 1 , wherein said at least one polysaccharide which is not (β-glucan is degraded to 1 DP.
19. The method of claim 1 , wherein after filtration the filtrate is diluted or concentrated so as to provide a mixture with a concentration of (β-glucan of 2% w/w.
20. The method of claim 1 , wherein after filtration the filtrate is diluted or concentrated so as to provide a mixture with a concentration of (β-glucan of 1% w/w.