Microscope system
Provided is a microscope system including: a galvanometer scanner that scans laser light on a sample; an observation optical system that acquires an image of the sample on which the laser light is scanned by the galvanometer scanner; an ROI setting portion that sets a region-of-note, which is an area of note in the sample, so as to serve as an observation ROI in the image acquired by the observation optical system; an amount-of-rotation calculating portion that calculates an amount by which the observation ROI is rotated about the Z-axis in the image in association with rotation of the region-of-note about the Z-axis; and a control portion that controls the galvanometer scanner so that the direction in which the laser light is scanned in the region-of-note is made steady on the basis of the amount by which the observation ROI is rotated about the Z-axis.
1. A microscope system comprising:
a scanning optical system that scans laser light on a sample;
an observation optical system that acquires an image of the sample on which the laser light is scanned by the scanning optical system;
a region-of-interest setting portion that sets a region-of-note, which is an area of note in the sample, so as to serve as a region-of-interest in the image acquired by the observation optical system;
an amount-of-rotation calculating portion that calculates an amount by which the region-of-interest is rotated about an observation optical axis in the image in association with rotation of the region-of-note about the observation optical axis of the observation optical system; and
a control portion that controls the scanning optical system so that the direction in which the laser light is scanned in the region-of-note is made steady on the basis of the amount by which the region-of-interest is rotated about the observation optical axis, which is calculated by the amount-of-rotation calculating portion.
2. A microscope system according to claim 1 , further comprising:
a focal-position moving portion that can move a focal position of the laser light in a direction along the observation optical axis; and
a first amount-of-movement calculating portion that calculates an amount by which the region-of-interest is moved in the direction along the observation optical axis in the image in association with movement of the region-of-note in the direction along the observation optical axis,
wherein the control portion causes the focal-position moving portion to move the focal position in the direction along the observation optical axis on the basis of the amount by which the region-of-interest is moved in the direction along the observation optical axis, which is calculated by the first amount-of-movement calculating portion.
3. A microscope system according to claim 2 , wherein, with respect to rotation of the region-of-interest associated with rotation of the region-of-note about an intersecting axis that intersects the observation optical axis, the amount-of-rotation calculating portion calculates an amount by which the region-of-interest is rotated about the intersecting axis in the image, and
the control portion causes the focal-position moving portion to move, in synchronization with scanning of the laser light by the scanning optical system, the focal position in the direction along the observation optical axis on the basis of the amount by which the region-of-interest is rotated about the intersecting axis, which is calculated by the amount-of-rotation calculating portion.
4. A microscope system according to claim 1 , further comprising:
a first amount-of-movement calculating portion that calculates an amount by which the region-of-interest is moved in a direction along the observation optical axis in the image in association with the movement of the region-of-note in the direction along the observation optical axis,
wherein the control portion causes the scanning optical system to move the focal position of the laser light in the direction along the observation optical axis on the basis of the amount by which the region-of-interest is moved in the direction along the observation optical axis, which is calculated by the first amount-of-movement calculating portion.
5. A microscope system according to claim 4 ,
wherein, with respect to rotation of the region-of-interest associated with rotation of the region-of-note about an intersecting axis that intersects the observation optical axis, the amount-of-rotation calculating portion calculates an amount by which the region-of-interest is rotated about the intersecting axis in the image, and
the control portion causes the scanning optical system to move, while scanning the laser light in a direction that intersects the observation optical axis, the focal position in the direction along the observation optical axis on the basis of the amount by which the region-of-interest is rotated about the intersecting axis, which is calculated by the amount-of-rotation calculating portion.
6. A microscope system according to claim 1 , further comprising:
a scanning-area moving portion that can move the area in which the laser light is scanned, by the scanning optical system, in two-dimensional directions that intersect the observation optical axis; and
a second amount-of-movement calculating portion that calculates amounts by which the region-of-interest is moved in the two-dimensional directions in the image in association with the movement of the region-of-note in the two-dimensional directions,
wherein the control portion causes the scanning-area moving portion to move the area in which the laser light is scanned in the two-dimensional directions on the basis of the amounts by which the region-of-interest is moved in the two-dimensional directions, which are calculated by the second amount-of-movement calculating portion.
7. A microscope system according to claim 1 , further comprising:
an additional scanning optical system that scans the laser light on the sample,
wherein the region-of-interest setting portion sets an additional region-of-note, which is an area of note in the sample, so as to serve as an additional region-of-interest in the image,
the amount-of-rotation calculating portion calculates an amount by which the additional region-of-interest is rotated about the observation optical axis in the image in association with rotation of the additional region-of-note about the observation optical axis, and
the control portion controls the additional scanning optical system so that the direction in which the laser light is scanned in the additional region-of-note is made steady on the basis of the amount by which the additional region-of-interest is rotated about the observation optical axis, which is calculated by the amount-of-rotation calculating portion.
8. A microscope system according to claim 7 , further comprising:
an additional focal-position moving portion that can move the focal position of the laser light in a direction along the observation optical axis; and
an additional first amount-of-movement calculating portion that calculates an amount by which the additional region-of-interest is moved in the direction along the observation optical axis in the image in association with movement of the additional region-of-note in the direction along the observation optical axis,
wherein the control portion causes the additional focal-position moving portion to move the focal position in the direction along the observation optical axis on the basis of the amount by which the additional region-of-interest is moved in the direction along the observation optical axis, which is calculated by the additional first amount-of-movement calculating portion.
9. A microscope system according to claim 8
wherein, with respect to rotation of the additional region-of-interest associated with rotation of the additional region-of-note about an intersecting axis that intersects the observation optical axis, the amount-of-rotation calculating portion calculates an amount by which the additional region-of-interest is rotated about the intersecting axis in the image, and
the control portion causes the additional focal-position moving portion to move, in synchronization with scanning of the laser light by the additional scanning optical system, the focal position in the direction along the observation optical axis on the basis of the amount by which the additional region-of-interest is rotated about the intersecting axis, which is calculated by the amount-of-rotation calculating portion.
10. A microscope system according to claim 7 , further comprising:
an additional first amount-of-movement calculating portion that calculates an amount by which the additional region-of-interest is moved in a direction along the observation optical axis in the image in association with the movement of the additional region-of-note in the direction along the observation optical axis,
wherein the control portion causes the additional scanning optical system to move the focal position of the laser light in the direction along the observation optical axis on the basis of the amount by which the additional region-of-interest is moved in the direction along the observation optical axis, which is calculated by the additional first amount-of-movement calculating portion.
11. A microscope system according to claim 10 ,
wherein, with respect to rotation of the additional region-of-interest associated with rotation of the additional region-of-note about an intersecting axis that intersects the observation optical axis, the amount-of-rotation calculating portion calculates an amount by which the additional region-of-interest is rotated about the intersecting axis in the image, and
the control portion causes the additional scanning optical system to move, while scanning the laser light by the additional scanning optical system in the direction that intersects the observation optical axis, the focal position in the direction along the observation optical axis on the basis of the amount by which the additional region-of-interest is rotated about the intersecting axis, which is calculated by the amount-of-rotation calculating portion.
12. A microscope system according to claim 7 , further comprising:
an additional scanning-area moving portion that can move the area in which the laser light is scanned, by the additional scanning optical system, in two-dimensional directions that intersect the observation optical axis; and
an additional second amount-of-movement calculating portion that calculates an amount by which the additional region-of-interest is moved in the two-dimensional directions in the image in association with the movement of the additional region-of-note in the two-dimensional directions,
wherein the control portion causes the additional scanning-area moving portion to move, in the two-dimensional directions, the area in which the laser light is scanned on the basis of the amounts by which the additional region-of-interest is moved in the two-dimensional directions, which are calculated by the additional second amount-of-movement calculating portion.