Recovery and processing of human embryos formed in vivo
A kit having instructions for use for performing uterine lavage in a female patient includes a uterine lavage catheter configured for insertion into a woman's uterus to remove viable blastocysts from the uterus, and one or more first containers having a sufficient dosage amount of a GnRH antagonist to cause desynchronization of the endometrium of the patient prior to, during and/or following recovery of viable blastocysts from the uterus.
1. A method comprising:
providing a fluid delivery and collection device;
programming a controller to deliver lavage liquid from the fluid delivery and collection device to a uterus to assist with recovery of blastocysts from the uterus at a flow of fluid supply that substantially limits or prevents leakage of fluid into the fallopian tubes by limiting total volume of liquid in the uterus at any given time to not exceed 10 mL;
performing a biopsy procedure on the recovered blastocysts;
adding to, replacing, or manipulating a dysfunctional sequence of DNA from the recovered blastocysts by removing one or more stem cells from an inner cell mass of the biopsied blastocysts and transfecting the one or more stem cells directly using a retroviral vector or by micro insertion of a gene construct into the one or more stem cells; and
reintroducing the transfected one or more stem cells into the inner cell mass of the recovered blastocysts.
2. The method of claim 1 , further comprising programming the controller to control the flow of fluid supply such that it does not exceed a maximum pressure of the device of between about 2 ounces per square inch and 50 pounds of pressure per square inch.
3. The method of claim 1 , further comprising programming the controller to control the flow of fluid supply such that it does not exceed a maximum pressure of the device of between about 2 ounces per square inch and 20 pounds of pressure per square inch.
4. The method of claim 1 , further comprising programming the controller to control the delivery of the fluid through the fluid delivery and collection device into the uterus in a series of pulses at a pre-determined pulse rate.
5. The method of claim 4 , wherein the pulse rate has a preset frequency in the range of one pulse per 0.5 to 4 seconds.
6. The method of claim 1 , wherein the controller is programmed to automatically control the delivery of the fluid through the fluid delivery and collection device into the uterus.
7. A method comprising:
providing a fluid delivery and collection device;
programming a controller to deliver lavage liquid in a series of pulses at a first pre-determined pulse rate to a uterus from the fluid delivery and collection device and to apply vacuum pressure to liquid in the uterus in a series of vacuum pulses at a second pre-determined pulse rate to assist with recovery of blastocysts from the uterus while maintaining integrity of the blastocysts during fluid delivery and removal and limiting total volume of liquid in the uterus at any given time to not exceed 10 mL;
performing a biopsy procedure on the recovered blastocysts;
adding to, replacing, or manipulating a dysfunctional sequence of DNA from the recovered blastocysts by removing one or more stem cells from an inner cell mass of the biopsied blastocysts and transfecting the one or more stem cells directly using a retroviral vector or by micro insertion of a gene construct into the one or more stem cells; and
reintroducing the transfected one or more stem cells into the inner cell mass of the recovered blastocysts.
8. The method of claim 7 , wherein the vacuum pressure is between about 10 to 14 Hg.
9. The method of claim 7 , further comprising programming the controller to control withdrawal of the lavage liquid through the fluid delivery and collection device from the uterus in a series of vacuum pulses at a pre-determined pulse rate.
10. The method of claim of claim 7 , wherein the controller is programmed to automatically control the delivery of the lavage liquid through the fluid delivery and collection device into the uterus.
11. A method of performing uterine lavage comprising:
delivering lavage liquid from a fluid delivery and collection device to the uterus at a flow of fluid supply that substantially limits or prevents leakage of fluid into the fallopian tubes by limiting total volume of liquid in the uterus at any given time to not exceed 10 mL;
recovering blastocysts from the uterus;
performing a biopsy procedure on the recovered blastocysts;
adding to, replacing, or manipulating a dysfunctional sequence of DNA from the recovered blastocysts by removing one or more stem cells from an inner cell mass of the biopsied blastocysts and transfecting the one or more stem cells directly using a retroviral vector or by micro insertion of a gene construct into the one or more stem cells; and
reintroducing the transfected one or more stem cells into the inner cell mass of the recovered blastocysts.
12. A method of performing uterine lavage comprising:
delivering lavage liquid from a fluid delivery and collection device to the uterus;
applying vacuum pressure to liquid in the uterus;
recovering blastocysts from the uterus while maintaining integrity of the blastocysts during fluid delivery and removal and limiting total volume of liquid in the uterus at any given time to not exceed 10 mL;
performing a biopsy procedure on the recovered blastocysts;
adding to, replacing, or manipulating a dysfunctional sequence of DNA from the recovered blastocysts by removing one or more stem cells from an inner cell mass of the biopsied blastocysts and transfecting the one or more stem cells directly using a retroviral vector or by micro insertion of a gene construct into the one or more stem cells; and
reintroducing the transfected one or more stem cells into the inner cell mass of the recovered blastocysts.