IP Library Granted Patent US 9,956,534
Granted Patent B2
US 9,956,534 · App. 15/676,048 · Granted May 1, 2018

Method and device for suspending cells

Inventors: Dominik Hammerschmidt (Lindlar, DE); Sven Ignatius (Bergisch Gladbach, DE); Martin Buscher (Bergische Gladbach, DE); Ralf-Peter Peters (Bergisch Gladbach, DE)
Assignee: Miltenyi Biotec, GmbH
B01F13/0854B01F11/0082B01F11/0091B01F13/08B01F13/0827B01F15/00032B01F15/0203C12M27/02G01N1/38B01F2215/0037G01N2001/386G01N2035/1058
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Quick Facts
Patent No.
US 9,956,534
App. No.
15/676,048
Granted
May 1, 2018
Kind
B2
Abstract

The invention is related to a device for suspending particles in a fluid, wherein the mixing device includes a first magnet ( 1 ) rotating around a longitudinal axis ( 2 ), a mixing rod ( 4 ) attached to a mount ( 6 ), the mount including a second magnet ( 3 ), wherein the mount ( 6 ) moves in a substantially orthogonal motion to the longitudinal axis of the mixing rod ( 4 ) by the interaction of the rotating first magnet with the second magnet ( 3 ).

Claims (16)

1. A mixing device comprising:

a first magnet ( 1 ) rotating around a longitudinal axis ( 2 );

a mixing rod ( 4 ) attached to a mount ( 6 ), the mount including a second magnet ( 3 ), wherein the mount ( 6 ) moves in a substantially orthogonal motion to the longitudinal axis ( 2 ) of the mixing rod ( 4 ) by an interaction of the rotating first magnet with the second magnet ( 3 ) wherein the orthogonal motion is accommodated by a plurality of joints disposed on at least two sides of the mount ( 6 ) and wherein the plurality of joints allows movement of the second magnet ( 3 ) only about a second axis which is orthogonal to the longitudinal axis ( 2 ).

2. The mixing device according to claim 1 , wherein the longitudinal axis ( 2 ) of the first magnet is substantially coaxial with a rotational axis of the second magnet ( 3 ).

3. The mixing device according to claim 1 , characterized in that the mixing rod is a tube.

4. The mixing device according to claim 1 , characterized in that the mixing rod ( 4 ) is coupled to the mount ( 6 ) by a sleeve ( 10 ), wherein the sleeve allows movement of the mixing rod along its longitudinal axis.

5. The mixing device according to claim 1 , characterized in that the mount ( 6 ) is provided with at least one orifice ( 11 , 12 ) which allows introduction of a liquid into a sleeve ( 10 ).

6. The mixing device according to claim 1 , characterized in that the mount ( 6 ) is provided with at least a cleaning pod that removes material from the mixing rod.

7. The mixing device according to claim 1 , characterized in that at least one mixing vessel containing a liquid is provided below the mount ( 6 ), such that the mixing rod is submerged in the liquid so as to stir the liquid by the orthogonal motion.

8. The mixing device according to claim 1 , characterized in that at least one mixing vessel is provided and the mixing rod ( 4 ) is inserted and removed from the mixing vessel by moving the mixing vessel in a direction along the longitudinal axis of the mixing rod ( 4 ).

9. The mixing device according to claim 1 , wherein the mixing rod does not touch walls of a mixing vessel throughout its movement.

10. The mixing device according to claim 7 , wherein the outer diameter of the mixing rod ( 4 ) is between about 0.1 and 0.3 of a diameter of the vessel.

11. The mixing device according to claim 1 , further comprising an additional 1 to 6 mixing rods ( 4 ) and mounts ( 10 ) which are driven in parallel.

12. The mixing device according to claim 1 , wherein the mount ( 6 ) includes a plurality of mixing rods ( 4 ).

13. The mixing device according to claim 1 , further comprising a driver that stops and reverses motion of the mixing rod.

14. The mixing device according to claim 1 , wherein the mixing rod ( 4 ) mixes a biological sample.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 29, 2019
From: MILTENYI BIOTEC GMBH
To: MILTENYI BIOTEC B.V. & CO. KG
Reel/Frame 051443/0669 →
Priority Claims (1)
EP 14168756 · May 17, 2014 · regional
Continuity (2)
Continuation In Part 14705037 · May 6, 2015
Related Publication 20170361289A1 · Dec 21, 2017