Primer pair, kit and method for detecting
View Patent ↗Primer pair, kit and method for detecting Ehrlichia canis are disclosed. The primer pair includes a forward primer and a reverse primer, and the kit includes the primer pair and a probe. The forward primer has a sequence of SEQ ID NO: 1, the reverse primer has a sequence of SEQ ID NO: 2, and the probe has a sequence of SEQ ID NO: 3.
1. A method for detecting Ehrlichia canis , the method comprising amplifying nucleic acid from Ehrlichia canis using real-time polymerase chain reaction with a forward primer consisting of a sequence of 5′-ATTAATGTACTATGCTCCAAG-3′ (SEQ ID NO: 1) and a reverse primer consisting of a sequence of 5′-GTTGAGTTTCTTTCTTCTTTAG-3′ (SEQ ID NO: 2).
2. The method according to claim 1 wherein a probe consisting of a sequence of 5′-ACTATACAAGACGATAACACTGGTAGC-3′ (SEQ ID NO: 3) is used for the real-time polymerase chain reaction.
3. The method according to claim 2 wherein the probe is labeled with a 5′-reporter dye and a 3′-quencher.