Microscope including a medium container containing an immersion medium in which a specimen container containing an immersion medium and a sample is immersed
Provided is a microscope including: a chamber storing a solution in which a cuvette accommodating a solution together with a sample is immersed and that has an index of refraction identical to that of the solution; an immersion objective lens being placed outside the chamber and collecting light from the sample; a camera acquiring an image of the light collected by the lens; a targeting section moving the lens in a direction along a detection light axis thereof; and a movable stage supporting the cuvette in the chamber so as to be movable in at least a direction along the detection light axis. Each of the cuvette and the chamber has a transparent section that can transmit light coming from the sample. The lens is placed so as to face the transparent section of the cuvette with the transparent section of the chamber interposed therebetween.
1. A microscope comprising:
a medium container that stores a second liquid immersion medium in which a specimen container accommodating a first liquid immersion medium together with a specimen is immersed, the second liquid immersion medium having an index of refraction identical to that of the first liquid immersion medium;
an objective lens that is placed outside the medium container and that collects light emitted from the specimen;
an image-capturing unit that acquires an image of the light collected by the objective lens;
a targeting section that moves a focal position of the objective lens in a direction along a detection light axis thereof; and
a movable stage that supports the specimen container in the medium container such that the specimen container can move at least in a direction along the detection light axis,
wherein each of the specimen container and the medium container has a light-transmitting section capable of transmitting the light from the specimen, and
wherein the objective lens is disposed so as to face the light-transmitting section of the specimen container, with the light-transmitting section of the medium container interposed therebetween.
2. The microscope according to claim 1 , wherein the objective lens is disposed with a space interposed between the objective lens and the light-transmitting section of the medium container.
3. The microscope according to claim 1 , wherein the objective lens is an immersion objective lens, and the immersion objective lens is disposed with a third liquid immersion medium interposed between the immersion objective lens and the light-transmitting section of the medium container.
4. The microscope according to claim 3 , wherein:
the immersion objective lens is disposed with the detection light axis oriented in a direction intersecting a vertical direction, and
the third liquid immersion medium is held between the immersion objective lens and the light-transmitting section of the medium container due to surface tension.
5. The microscope according to claim 4 , wherein:
the specimen container and the medium container have the light-transmitting sections at side wall sections, and
the immersion objective lens is disposed with the detection light axis oriented in a direction substantially orthogonal to the vertical direction.
6. The microscope according to claim 3 , wherein the third liquid immersion medium has an index of refraction identical to that of the second liquid immersion medium.
7. The microscope according to claim 1 , wherein the light-transmitting section of the specimen container has an index of refraction identical to that of the second liquid immersion medium.
8. The microscope according to claim 1 , wherein the light-transmitting section of the medium container has an index of refraction identical to that of the second liquid immersion medium.
9. The microscope according to claim 1 , wherein the movable stage supports the specimen container so as to be movable in a direction intersecting the detection light axis.
10. The microscope according to claim 1 , wherein the image-capturing unit acquires an image of the light self-emitted by the specimen.
11. The microscope according to claim 1 , further comprising:
an illumination optical system that irradiates the specimen with light from a direction intersecting the detection light axis,
wherein each of the specimen container and the medium container has a light transmitting section that transmits, towards the specimen, the light coming from the illumination optical system.
12. The microscope according to claim 11 , wherein:
the medium container has the light transmitting section, which transmits the light coming from the illumination optical system, at a bottom section, and
the illumination optical system includes a reflection mirror placed in the medium container, causes the light to enter the medium container via the light transmitting section at the bottom section, and reflects the light at the reflection mirror towards the specimen.
13. The microscope according to claim 11 , wherein:
the medium container has the light transmitting section, which transmits the light coming from the illumination optical system, at a side wall section, and
the illumination optical system causes the light to enter the medium container via the light transmitting section at the side wall section.
14. The microscope according to claim 11 , wherein the illumination optical system includes a lens that is placed in the medium container and that has positive refractive power.
15. The microscope according to claim 14 , wherein the lens having positive refractive power is a cylindrical lens having positive refractive power in one direction intersecting an illumination light axis of the illumination optical system.
16. The microscope according to claim 11 , further comprising:
a microlens array formed by two-dimensionally arranging a plurality of microlenses in directions intersecting an imaging light axis of the image-capturing unit,
wherein the illumination optical system causes the light in a form of a substantially collimated beam to enter the specimen.
17. The microscope according to claim 11 , wherein the movable stage supports a plurality of the specimen containers so as to be movable in the medium container about an axis parallel to the detection light axis.
18. The microscope according to claim 11 , wherein:
the specimen container has a plurality of specimen accommodation sections arranged in one direction intersecting an illumination light axis of the illumination optical system, and
the movable stage supports the specimen container so as to be capable of switching the specimen accommodation section placed on the detection light axis.
19. The microscope according to claim 11 , wherein the movable stage supports the specimen container so as to be rotatable in the medium container about the detection light axis.
20. The microscope according to claim 11 , wherein the illumination optical system causes the light in a flat shape to enter the specimen along a plane intersecting the detection light axis of the objective lens.