Methods and Compositions for Selecting siRNA of Improved Functionality
Efficient sequence specific gene silencing is possible through the use of siRNA technology. Be selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods compositions, and kits generated through rational design of siRNAs are disclosed, including those directed to the nucleotide sequences for F12.
1 . A polynucleotide comprising one of SEQ ID NO: 438-508 for use in gene silencing applications.
2 . A polynucleotide complementary to the polynucleotide of claim 1 for use in gene silencing applications.
3 . A composition comprising the polynucleotide of claim 1 or claim 2 .
4 . An siRNA molecule comprising the polynucleotide of claim 1 or claim 2 , wherein the siRNA molecule comprises a sense strand and an antisense strand.
5 . The siRNA molecule of claim 4 , wherein the siRNA molecule consists of: (a) a duplex region; and (b) either no overhang regions or at least one overhang region, wherein each overhang region contains six or fewer nucleotides, wherein the duplex region consists of a sense region and an antisense region, wherein the sense region and the antisense region together form the duplex region and the sense region and the antisense region are each 19-30 nucleotides in length and the antisense region comprises a sequence that is the complement of SEQ ID NO: 438-508.
6 . The siRNA molecule of claim 5 , wherein the antisense region and the sense region are each 19-25 nucleotides in length.
7 . The siRNA molecule of claim 6 , wherein the antisense region and the sense region are each 19 nucleotides in length.
8 . The siRNA molecule of claim 5 , wherein the siRNA molecule has at least one overhang region.
9 . The siRNA molecule of claim 5 , wherein the siRNA molecule has no overhang regions.
10 . The siRNA molecule of claim 6 , wherein the siRNA molecule has at least one overhang region.
11 . The siRNA molecule of claim 6 , wherein the siRNA molecule has no overhang regions.
12 . The siRNA molecule of claim 7 , wherein the siRNA molecule has at least one overhang region.
13 . The siRNA molecule of claim 7 , wherein the siRNA molecule has no overhang regions.
14 . The antisense strand of the siRNA molecule of claim 4 .
15 . The sense strand of the siRNA molecule of claim 4 .