Microorganisms engineered to use unconventional sources of nitrogen
Disclosed are genetically engineered organisms, such as yeast and bacteria, that have the ability to metabolize atypical nitrogen sources, such as melamine and cyanamide. Fermentation methods using the genetically engineered organisms are also described. The methods of the invention are robust processes for the industrial bioproduction of a variety of compounds, including commodities, fine chemicals, and pharmaceuticals.
1. A fermentation method of culturing a genetically engineered microbial a cell comprising:
providing a cell comprising a non-native nucleic acid molecule encoding a melamine deaminase enzyme:
contacting the cell with a substrate, wherein the substrate comprises a nitrogen-containing fraction and a non-nitrogen-containing fraction and wherein the nitrogen-containing fraction comprises, in an amount from about 10% by weight to about 100% by weight, a nitrogen-containing compound of Formula I, or a salt thereof; and
culturing the cell, wherein the cell can, unlike a cell of the same species that lacks the nucleic acid molecule, metabolize the nitrogen-containing compound and wherein,
the compound of formula I is
wherein, independently for each occurrence,
is a five-, or a six-membered aryl or heteroaryl group;
R is —OH, —CO 2 H, —NO 2 , —CN, substituted or unsubstituted amino, or substituted or unsubstituted alkyl; and
n is 0, 1, 2, 3, 4, or 5.
2. The method of claim 1 , wherein the nitrogen-containing compound is selected from the group consisting of 5-Aminotetrazole, Melamine, Ammeline, and Ammelide.
3. The method of claim 1 , wherein the substrate does not comprise an antibiotic.
4. The method claim 1 , wherein the substrate does not comprise ammonium or urea.
5. The method of claim 1 , wherein the substrate comprises lignocellulosic material, glucose, xylose, sucrose, acetic acid, formic acid, lactic acid, butyric acid, a free fatty acid, dextrose, glycerol, fructose, lactose, galactose, mannose, rhamnose, or arabinose, or a combination thereof.
6. The method of claim 1 , wherein the genetically engineered cell is contacted with the substrate in a fermentor.
7. The method of claim 1 , wherein the non-native nucleic acid molecule comprises SEQ ID NO: 12 or SEQ ID NO: 13 or the non-native molecule comprises a sequence having at least 85% sequence identity with SEQ ID NO:12 or SEQ ID NO:13.