Device and method for observing and filter for capturing a minute substance
There is provided a glass substrate for observing minute substance, made of porous glass and capable of separating and capturing a minute substance with a 10 to 500 nm average particle diameter contained in a solution or a suspension, comprising a porous glass substrate having a plurality of pores, wherein the plurality of pores has an average pore diameter ranging from 30 to 110% of the average particle diameter of the minute substance, each of the plurality of pores has a surface pore diameter on an uppermost surface of the glass substrate, a standard deviation of the surface pore diameter is 60% or less of the average particle diameter of the minute substance, and a pore with a pore diameter ranging from 60 to 140% of a pore diameter at peak top in a pore diameter distribution of the plurality of pores occupies 90% or more of total pore volume.
1. A filter for capturing a minute substance, comprising a porous glass substrate having a plurality of pores,
wherein
the porous glass substrate comprises phase-separated glass formed through a spinodal decomposition,
the plurality of pores has an average pore diameter ranging from 5 to 2500 nm and a pore diameter ranging from 60 to 140% of a pore diameter corresponding to a maximum value in a pore diameter distribution of the plurality of pores occupies 90% or more of total pore volume of the plurality of pores, and
the porous glass substrate satisfies the following formula (1):
S
D
·
log
10
d
≤
4.5
[
cm
2
/
mm
]
(
1
)
wherein S [cm 2 ] is an area of main surface of the glass substrate,
D [mm] is a plate thickness of the porous glass substrate, and
d [nm] is the average pore diameter.
2. The filter for capturing a minute substance according to claim 1 , wherein the porous glass substrate has a porosity ranging from 20 to 90%.
3. A filter for capturing a minute substance, comprising a porous glass substrate having a plurality of pores,
wherein
the plurality of pores has an average pore diameter ranging from 5 to 2500 nm and a pore diameter ranging from 60 to 140% of a pore diameter corresponding to a maximum value in a pore diameter distribution of the plurality of pores occupies 90% or more of total pore volume of the plurality of pores, and
the porous glass substrate has a covering layer on a surface, the covering layer comprising a protein anti-adhesion agent.
4. A minute substance observation device comprising:
a capturing unit including the filter according to claim 1 and being capable of separating and capturing a minute substance with an average particle diameter of 10 to 500 nm contained in a solution or a suspension; and
an observing unit for observing the minute substance captured on a surface of the glass substrate.
5. The minute substance observation device according to claim 4 , wherein the observing unit is a fluorescence microscope.
6. A minute substance capturing method comprising:
introducing a solution or a suspension comprising a minute substance with a 10 to 500 nm average particle diameter to a porous glass substrate having a plurality of pores; and
separating and capturing the minute substance by the glass substrate,
wherein
the porous glass substrate comprises phase-separated glass formed through a spinodal decomposition,
the plurality of pores has an average pore diameter ranging from 30 to 500% of the average particle diameter of the minute substance, and a pore diameter ranging from 60 to 140% of a pore diameter corresponding to a maximum value in a pore diameter distribution of the plurality of pores occupies 90% or more of total pore volume of the plurality of pores.
7. The minute substance capturing method according to claim 6 , wherein the porous glass substrate satisfies the following formula (1):
S
D
·
log
10
d
≤
4.5
[
cm
2
/
mm
]
,
(
1
)
wherein
S [cm 2 ] is an area of main surface of the glass substrate,
D [mm] is a plate thickness of the porous glass substrate, and
d [nm] is the average pore diameter.
8. A minute substance observation method comprising:
introducing a solution or a suspension comprising a minute substance with a 10 to 500 nm average particle diameter to a porous glass substrate having a plurality of pores;
separating and capturing the minute substance by the glass substrate; and
observing the minute substance captured by the glass substrate,
wherein
an average pore diameter ranging from 30 to 110% of the average particle diameter of the minute substance,
each of the plurality of pores has a surface pore diameter on a main surface of the porous glass substrate, and a standard deviation of the surface pore diameter on an uppermost surface of the glass substrate is 60% or less of the average particle diameter of the minute substance, and
a pore with a pore diameter ranging from 60 to 140% of a pore diameter corresponding to a maximum value in a pore diameter distribution of the plurality of pores occupies 90% or more of total pore volume of the plurality of pores.
9. The minute substance observation method according to claim 8 , wherein the average pore diameter is ranging from 30 to 100% of the average particle diameter of the minute substance.
10. A minute substance observation method comprising:
separating and capturing a minute substance with a 10 to 500 nm average particle diameter by the filter according to claim 1 , from a solution or a suspension comprising the minute substance; and
observing the minute substance captured on a surface of the glass substrate for observing minute substance.
11. The minute substance observation method according to claim 8 , wherein a fluorescence microscope is used for the observation.