ALZHEIMER'S DISEASE DIAGNOSTIC PANELS AND METHODS FOR THEIR USE
Novel compositions, methods, assays and kits directed to a diagnostic panel for Alzheimer's disease are provided. In one embodiment, the diagnostic panel includes one or more proteins associated with Alzheimer's disease.
1 . A method for diagnosing Alzheimer's disease in a subject, comprising:
determining the protein expression of a plurality of proteins comprising at least F13A1, PON1, ITIH1, CLU, APOD, GSN, and APOA4 from a biological sample from the subject;
comparing the protein expression from step (a) to the protein expression of a plurality of proteins comprising at least F13A1, PON1, ITIH1, CLU, APOD, GSN, and APOA4 from a control biological sample, wherein the control biological sample is obtained from a subject with cognitive impairment due to the normal effects of aging or with no cognitive impairment;
diagnosing Alzheimer's disease in the subject based on the differential protein expression of the plurality of proteins between the subject biological sample and the control biological sample, wherein the subject is diagnosed with Alzheimer's disease if the differential protein expression has a statistical p value of 0.05 or below.
2 . The method of claim 1 , wherein diagnosing Alzheimer's disease occurs at least two years before the subject experiences the onset of mild to moderate cognitive impairment.
3 . The method of claim 1 , wherein diagnosing of Alzheimer's disease occurs at least four years before the subject experiences the onset of mild to moderate cognitive impairment.
4 . The method of claim 1 , further comprising administering a mini-mental state examination (MMSE) to the subject.
5 . The method of claim 5 , wherein diagnosing Alzheimer's disease in further comprises determining whether the subject has a MMSE score of less than 26.
6 . The method of claim 6 , wherein the subject has an MMSE score of less than 21.
7 . The method of claim 7 , wherein the subject has an MMSE score of less than 10.
8 . The method of claim 1 , wherein the biological sample is blood, plasma or a serum sample.
9 . The method of claim 1 , wherein protein expression can be determined by reverse transcriptase-polymerase chain reaction (RT-PCR), microarray, serial analysis of gene expression (SAGE), gene expression analysis by massively parallel signature sequencing (MPSS), immunoassays, immunohistochemistry (IHC), mass spectrometry (MS), transcriptomics, or proteomics.
10 . The method of claim 10 , wherein the mass spectrometry is chromatography-mass spectrometry (LC-MS) using eXtracted Ion Chromatograms (XIC), selected ion monitoring (SIM), selected reaction monitoring (SRM), multiple reaction monitoring mass spectrometry (MRM), or MRM-triggered MS/MS (MRM-MS/MS).
11 . The method of claim 1 , further comprising detecting one or more peptide transitions of the plurality of proteins, the peptide transitions comprising at least SEQ ID NO: 1 (LIASMSSDSLR (590.3-1066.3)), SEQ ID NO: 3 (IQNILTEEPK (592.8-829.4)), SEQ ID NO: 1 (LIASMSSDSLR (590.3-953.2)), SEQ ID NO: 3 (IQNILTEEPK (592.8-943.4)), SEQ ID NO: 4 (GSLVQASEANLQAAQDFVR (1002.5-1448.6)), SEQ ID NO: 5 (EIQNAVNGVK (536.3-417.2)), SEQ ID NO: 6 (VLNQELR (436.2-659.3)), SEQ ID NO: 4 (GSLVQASEANLQAAQDFVR (1002.5-1232.6)), SEQ ID NO: 7 (TGAQELLR (444.2-530.3)), SEQ ID NO: 8 (ALVQQMEQLR (608.3-932.5)), SEQ ID NO: 7 (TGAQELLR (444.2-658.4)), SEQ ID NO: 9 (ELDESLQVAER (644.8-802.4)), SEQ ID NO: 6 (VLNQELR (436.2-772.4)), or SEQ ID NO: 10 (EVAFDLEIPK (580.8-861.5)).
12 . The method of claim 1 , wherein the plurality of proteins comprises at least A1BG, APOA4, APOD, ARSA, ATP2A2, BDNF, CACNB2, CALML3, CDH5, CLU, COL18A1, COL1A2, CPN1, CSF1R, EPB41, EPHA8, F13A1, GALR3, CG, GNAQ, GPR113, GRIN2A, GRN, GSN, HPX, INADL, ITIH1, ITIH2, Kng1, LAMB2, LRP8, LTBP1, MMP16, MPDZ, MTOR, NMB, NTRK2, PACSIN1, PARD3, PKDREJ, PON1, PTPRB, SEMG1, SERPINA3, SERPINA4, SERPINF1, SNCB, SYTL4, TMPRSS2 and VTN.
13 . A kit comprising reagents for determining the protein expression of a plurality of proteins comprising at least F13A1, PON1, ITIH1, CLU, APOD, GSN, and APOA4 from a biological sample from a subject and instructions for performing the method of claim 1 .
14 . The kit of claim 14 , comprising reagents for detecting one or more peptide transitions of the plurality of proteins.
15 . A kit of claim 15 , comprising reagents for detecting one or more peptide transitions of the plurality of proteins, the peptide transitions comprising at least SEQ ID NO: 1 (LIASMSSDSLR (590.3-1066.3)), SEQ ID NO: 3 (IQNILTEEPK (592.8-829.4)), SEQ ID NO: 1 (LIASMSSDSLR (590.3-953.2)), SEQ ID NO: 3 (IQNILTEEPK (592.8-943.4)), SEQ ID NO: 4 (GSLVQASEANLQAAQDFVR (1002.5-1448.6)), SEQ ID NO: 5 (EIQNAVNGVK (536.3-417.2)), SEQ ID NO: 6 (VLNQELR (436.2-659.3)), SEQ ID NO: 4 (GSLVQASEANLQAAQDFVR (1002.5-1232.6)), SEQ ID NO: 7 (TGAQELLR (444.2-530.3)), SEQ ID NO: 8 (ALVQQMEQLR (608.3-932.5)), SEQ ID NO: 7 (TGAQELLR (444.2-658.4)), SEQ ID NO: 9 ELDESLQVAER (644.8-802.4), SEQ ID NO: 6 (VLNQELR (436.2-772.4)), or SEQ ID NO: 10 (EVAFDLEIPK (580.8-861.5)).