T cell balance gene expression, compositions of matters and methods of use thereof
This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.
1. A method of discovering a compound capable of altering an immune response in Th17 cells comprising the steps of:
(a) providing a compound or plurality of compounds;
(b) providing a population of Th17 cells which express Gpr65, Plzp, and/or Toso, and does not express Cd5l;
(c) contacting said compound or plurality of compounds with said population of cells;
(d) detecting a level of expression of Gpr65, Plzp, and/or Toso, and Cd5l;
(e) comparing the detected level to a control level of a population of Th17 cells not contacted with said compound or plurality of compounds; and
(f) evaluating the difference between the detected level and the control level to determine if the compound or plurality of compounds alters an immune response in Th17 cells.
2. The method of claim 1 , wherein a compound or plurality of compounds that decreases expression of Gpr65, Plzp, and/or Toso, and increases expression of Cd5l is discovered.
3. The method of claim 1 , wherein the population of Th17 cells is contacted in vitro or ex vivo.
4. The method of claim 1 , wherein the population of Th17 cells are obtained from a subject having an autoimmune response or an inflammatory response.
5. The method of claim 4 , wherein the autoimmune response is selected from the group consisting of multiple sclerosis (MS), psoriasis, inflammatory bowel disease, ulcerative colitis, Crohn's disease, uveitis, lupus, ankylosing spondylitis, and rheumatoid arthritis.