Nucleotide sequences and corresponding polypeptides conferring modified phenotype characteristics in plants
Methods and materials for modulating low-nitrogen tolerance levels in plants are disclosed. For example, nucleic acids encoding low nitrogen tolerance-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased [RCL2] low-nitrogen tolerance levels and plant products produced from plants having increased low-nitrogen tolerance levels.
1. A method of producing a plant, said method comprising growing a plant cell comprising an exogenous nucleic acid molecule, said exogenous nucleic acid molecule comprising a regulatory region operably linked to a polynucleotide comprising a nucleic acid sequence having 95 percent or greater sequence identity to the nucleic acid sequence of SEQ ID NO:96, producing a plant from said plant cell, and selecting a plant for an increased level of low-nitrogen tolerance as compared to the level of low-nitrogen tolerance of a control plant that does not comprise said nucleic acid molecule.
2. The method of claim 1 , wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO:96.
3. A method of increasing a level of low-nitrogen tolerance in a plant, said method comprising introducing into a plant cell an exogenous nucleic acid molecule, said exogenous nucleic acid molecule comprising a regulatory region operably linked to a polynucleotide comprising a nucleic acid sequence having 95 percent or greater sequence identity to the nucleic acid sequence of SEQ ID NO:96, producing a plant from said plant cell, and selecting a plant comprising a tissue that has an increased level of low-nitrogen tolerance as compared to the level of low-nitrogen tolerance of a control plant that does not comprise said nucleic acid molecule.
4. The method claim 1 , wherein said polynucleotide comprises a nucleic acid sequence having 97 percent or greater sequence identity to the nucleic acid sequence of SEQ ID NO:96.
5. The method of claim 3 , wherein the polynucleotide comprises a nucleic acid sequence that has 97 percent or greater sequence identity to the nucleic acid sequence of SEQ ID NO:96.
6. A plant cell comprising an exogenous nucleic acid molecule said exogenous nucleic acid molecule comprising regulatory region operably linked to a polynucleotide comprising a nucleic acid sequence having 95 percent or greater sequence identity to the nucleic acid sequence of SEQ ID NO:96, wherein a plant comprising said plant cell has an increased level of low-nitrogen tolerance as compared to the level of low-nitrogen tolerance of a control plant that does not comprise said nucleic acid molecule.
7. The plant cell of claim 6 , wherein the polynucleotide comprises a nucleotide sequence that has 97 percent or greater sequence identity to the nucleic acid sequence of SEQ ID NO:96.
8. A transgenic plant comprising the plant cell of claim 6 .
9. The transgenic plant of claim 8 , wherein said plant is a member of a species selected from the group consisting of Panicum virgatum (switchgrass), Sorghum bicolor ( Sorghum , sudangrass), Miscanthus giganteus ( Miscanthus ), Saccharum sp. (energycane), Populus balsamifera (poplar), Zea mays (corn), Glycine max (soybean), Brassica napus (canola), Triticum aestivum (wheat), Gossypium hirsutum (cotton), Oryza sativa (rice), Helianthus annuus (sunflower), Medicago sativa (alfalfa), Beta vulgaris (sugarbeet), and Pennisetum glaucum (pearl millet).
10. A product comprising plant tissue from the transgenic plant according to claim 9 .
11. A transgenic plant or a progeny thereof, wherein the transgenic plant is produced by the method of claim 1 , and wherein the progeny comprises the exogenous nucleic acid molecule.