Methods and devices for amplification of nucleic acid
The present invention relates to methods and devices for amplifying nucleic acid, and, in particular, amplifying so as to generate products on a surface without the use of emulsions. In a preferred embodiment, a plurality of groups of amplified product are generated on the surface, each group positioned in different (typically predetermined) locations on said surface so as to create an array.
1. A method of sequencing nucleic acid, comprising:
a) providing i) a plurality of different amplicons immobilized on a first surface enclosed in a chamber, wherein said amplicons are attached to the first surface through extension of the primers which were attached to the surface prior to amplification, said amplification performed without the use of emulsions, and ii) sequencing reagents, wherein said sequencing reagents comprise polymerase and a plurality of sequencing primers;
b) introducing said sequencing reagents into said chamber; and
c) performing sequencing by synthesis.
2. The method of claim 1 , wherein said surface is a glass surface.
3. The method of claim 1 , wherein said glass surface is a glass chip.
4. The method of claim 1 , wherein said glass surface is a glass slide.
5. The method of claim 1 , wherein said chamber is sealed by an adhesive gasket, and a second surface.
6. The method of claim 5 , wherein said adhesive gasket is sandwiched between said first and second surfaces.
7. The method of claim 1 , wherein said amplicon is immobilized in one or more channels.
8. The method of claim 7 , wherein said channels are created by said first surface and a second surface.
9. A method of sequencing nucleic acid, comprising:
a) providing i) a plurality of primers immobilized on a first surface enclosed in a chamber, ii) amplification reagents comprising polymerase and nucleic acid template; and iii) sequencing reagents;
b) introducing said amplification reagents into said chamber under conditions such that amplicons are generated on said first surface, said amplicons attached to the first surface through extension of the primers, said amplicons generated without the use of emulsions; and
c) introducing said sequencing reagents and performing sequencing by synthesis.
10. The method of claim 9 , wherein said surface is a glass surface.
11. The method of claim 10 , wherein said glass surface is a glass chip.
12. The method of claim 10 , wherein said glass surface is a glass slide.