Plant promoter for transgene expression
View Patent ↗This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Zea mays egg cell gene. Some embodiments relate to a promoter from a Zea mays egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3′ UTR from a Zea mays egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences.
1. A nucleic acid vector comprising a promoter operably linked to a heterologous coding sequence of:
a) a polylinker sequence;
b) a non- Zea mays egg cell transgene; or
c) a combination of a) and b);
wherein said promoter comprises a polynucleotide sequence that has at least 95% sequence identity with SEQ ID NO:1.
2. The nucleic acid vector of claim 1 , wherein said promoter is 1,500 bp in length.
3. The nucleic acid vector of claim 1 , wherein said promoter consists of a polynucleotide sequence that has at least 95% sequence identity with SEQ ID NO:1.
4. The nucleic acid vector of any one of claim 1 , further comprising a sequence encoding a selectable marker.
5. The nucleic acid vector of claim 1 , wherein said promoter is operably linked to a heterologous coding sequence.
6. The nucleic acid vector of claim 5 , wherein the heterologous coding sequence encodes a selectable marker, an insecticidal resistance protein, a small RNA molecule, a site specific nuclease protein, a herbicide tolerance protein, a nitrogen use efficiency protein, a water use efficiency protein, a nutritional quality protein or a DNA binding protein.
7. The nucleic acid vector of claim 1 , further comprising a 3′ untranslated polynucleotide sequence.
8. The nucleic acid vector of claim 1 , further comprising a 5′ untranslated polynucleotide sequence.
9. The nucleic acid vector of claim 1 , further comprising an intron sequence.
10. The nucleic acid vector of claim 1 , wherein said promoter drives embryonic cell preferred expression of the heterologous coding sequence.
11. A transgenic plant comprising a polynucleotide sequence that has at least 98% sequence identity with SEQ ID NO:1 operably linked to a heterologous coding sequence, wherein said polynucleotide sequence promotes expression of the heterologous coding sequence.
12. The transgenic plant of claim 11 , wherein said plant is selected from the group consisting of Zea mays , wheat, rice, sorghum , oats, rye, bananas, sugar cane, soybean, cotton, Arabidopsis , tobacco, sunflower, and canola.
13. The transgenic plant of claim 12 , wherein said plant is Zea mays.
14. The transgenic plant of claim 11 , further comprising a 3′ untranslated sequence.
15. The transgenic plant of claim 11 , wherein said heterologous coding sequence is preferentially expressed within embryonic cell tissue.
16. The transgenic plant of claim 11 , wherein said promoter is 1,500 bp in length.