IP Library Granted Patent US 11,447,532
Granted Patent B2
US 11,447,532 · App. 15/705,185 · Granted Sep 20, 2022

Long uniform recombinant protein fibers

Inventors: David Breslauer (San Francisco, CA); Joshua Kittleson (Pleasant Hill, CA); Loren Perelman (Oakland, CA); Lindsay Wray (Benicia, CA)
Assignee: BOLT THREADS, INC.
C07K14/43518C12N15/81D01B7/00D01F4/00D02G3/042D01D5/06D06M2101/12D10B2211/04
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Quick Facts
Patent No.
US 11,447,532
App. No.
15/705,185
Granted
Sep 20, 2022
Kind
B2
Abstract

The present disclosure provides improved long uniform recombinant protein fibers with desirable physical traits. The present disclosure also provides compositions derived from the long uniform recombinant protein fibers.

Claims (36)

1. A long uniform recombinant protein fiber, comprising:

a continuous fiber length of at least 600 m, wherein the mean properties along the length of the fiber comprise:

a tenacity greater than or equal to 12 cN/tex;

a linear density less than or equal to 6 dtex;

a coefficient of variation of tenacity less than 15%; and

a coefficient of variation of linear density less than 20%,

wherein the tenacity is measured using ASTM D3822-14, and the linear density is measured using ASTM D1577;

wherein the recombinant protein fiber comprises a protein sequence comprising repeat units, wherein

each repeat unit has at least 95% sequence identity to a sequence that comprises from 2 to 20 quasi-repeat units,

each quasi-repeat unit comprising a polypeptide of {GGY-[GPG-X 1 ] n1 -GPS-(A) n2 } (SEQ ID NO: 111), wherein for each quasi-repeat unit: X 1 is independently selected from the group consisting of SGGQQ (SEQ ID NO: 100), GAGQQ (SEQ ID NO: 101), GQGPY (SEQ ID NO: 102), AGQQ (SEQ ID NO: 103), and SQ; n1 is from 4 to 8; and n2 is from 6 to 10.

2. The recombinant protein fiber of claim 1 , wherein the length is at least 650 m.

3. The recombinant protein fiber of claim 1 , wherein the tenacity has a coefficient of variation less than 10% along the length.

4. The recombinant protein fiber of claim 1 , wherein the linear density has a coefficient of variation less than 15% along the length.

5. The recombinant protein fiber of claim 1 , wherein the mean elongation at break is greater than 25% and the elongation at break has a coefficient of variation of less than 35% along the length.

6. The recombinant protein fiber of claim 1 , wherein the mean initial modulus is greater than 480 cN/tex and the initial modulus has a coefficient of variation of less than 5% along the length.

7. The recombinant protein fiber of claim 1 , wherein the mean elongation is greater than 24% and the elongation has a coefficient of variation of less than 45% along the length.

8. The recombinant protein fiber of claim 1 , wherein the mean work of rupture is greater than 3 cN*cm and the work of rupture has a coefficient of variation of less than 50% along the length.

9. The recombinant protein fiber of claim 1 , wherein the mean force at rupture is greater than 7 cN and the force at rupture has a coefficient of variation less than 25% along the length.

10. The recombinant protein fiber of claim 1 , wherein the recombinant protein fiber is produced by wet spinning a dope comprising a recombinant protein powder dissolved in a spinning solution.

11. The recombinant protein fiber of claim 10 , wherein the recombinant protein powder is less than 65% proteinaceous block copolymer by mass.

12. The recombinant protein fiber of claim 1 , wherein n1 is from 4 to 5 for at least half of the quasi-repeat units.

13. The recombinant protein fiber of claim 1 , wherein n2 is from 5 to 8 for at least half of the quasi-repeat units.

14. The recombinant protein fiber of claim 1 , wherein the repeat unit comprises alanine-rich regions and glycine-rich regions, wherein:

the alanine-rich regions form a plurality of nanocrystalline beta-sheets; and

the glycine-rich regions form a plurality of beta-turn structures.

15. The recombinant protein fiber of claim 1 , wherein the repeat unit comprises SEQ ID NO: 1.

16. The recombinant protein fiber of claim 1 , wherein the linear density and the tenacity are measured using FAVIMAT fiber tensile test equipment model Favimat+ and Robot2.

17. A yarn comprising the recombinant protein fiber of claim 1 , wherein the yarn is a filament yarn.

18. A textile comprising the yarn of claim 17 , wherein the textile is a knitted textile.

19. The textile of claim 18 , wherein the textile is selected from the group consisting of a circular-knitted textile, a flat-knitted textile, and a warp-knitted textiles.

20. A textile comprising the yarn of claim 17 , wherein the textile is a woven textile.

21. The textile of claim 20 , wherein the textile is selected from the group consisting of a plain weave textile, a dobby weave textile, and a jacquard weave textile.

22. A textile comprising the yarn of claim 17 , wherein the textile is a non-woven textile.

23. The textile of claim 22 , wherein the textile is selected from the group consisting of a needle punched textile, a spunlace textile, a wet-laid textile, a dry-laid textile, a melt-blown textile, and a 3-D printed non-woven textile.

24. A yarn comprising the recombinant protein fiber of claim 1 , wherein the yarn is a spun yarn.

25. A yarn comprising the recombinant protein fiber of claim 1 , wherein the yarn is a blended yarn.

Assignments (2)
SECURITY INTEREST Recorded Oct 14, 2022
From: BOLT THREADS, INC.
To: GINKGO BIOWORKS, INC.
Reel/Frame 061430/0311 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 7, 2018
From: BRESLAUER, DAVID; KITTLESON, JOSHUA; PERELMAN, LOREN; WRAY, LINDSAY
To: BOLT THREADS, INC.
Reel/Frame 045137/0515 →
Continuity (2)
Provisional Application 62394683 · Sep 14, 2016
Related Publication 20180111970A1 · Apr 26, 2018