Tuberculosis biomarkers and uses thereof
The present application includes biomarkers, methods, devices, reagents, systems, and kits for the detection, treatment and diagnosis of tuberculosis (TB). In one aspect, the present application includes the identification of biomarkers that can be used alone or in various combinations for the detection of TB, including those set forth in Tables 1, 2, 4, 5, and 8 to 12. In another aspect, the application provides biomarkers that can be used alone or in various combinations to diagnose or prognose TB or follow treatment response. In another aspect, methods are provided for diagnosing TB in an individual, where the methods include detecting, in a biological sample from an individual, at least one biomarker value corresponding to at least one biomarker selected from the group of biomarkers provided in Tables 1, 2, 4, 5, and 8 to 12, wherein the individual is classified as having TB, or the likelihood of the individual having TB is determined, based on the at least one biomarker value.
1. A kit for identifying the tuberculosis (TB) status of an individual, the kit comprising four aptamers for detecting the level of the protein biomarkers kallistatin, Complement component 9, afamin, and TSP4 in a sample from an individual;
wherein the TB status of the individual is TB-positive or TB-negative; and
wherein, each aptamer has specific affinity for a different protein of the protein biomarkers.
2. The kit of claim 1 , wherein the aptamers are capable of binding a solid support or are bound to a solid support.
3. The kit of claim 1 further comprising a solid support.
4. The kit of claim 1 further comprising a labeling agent, wherein the labeling agent labels the aptamers.