Compositions on plasma-treated surfaces
The disclosure provides solid compositions such as lyophilisates adhered to surfaces such as plasma-treated surfaces and related methods, uses, kits, intermediates, starting materials, and downstream products.
1. A method of forming a reconstitution mixture in a well of a plastic multiwell plate, the method comprising dispensing a reconstitution liquid onto a solid composition adhered to the well,
wherein the well is a plastic well comprising walls and a bottom that have been plasma-treated,
wherein the solid composition is a lyophilisate formed by dispensing a solution into the well and lyophilizing the solution, and
wherein, before dispensing the reconstitution liquid, the solid composition is sufficiently adhered to the bottom and walls of the well so as to remain substantially attached to the well following a package performance test in which the plastic multiwell plate comprising the well containing the solid composition is dropped from a height of about 20 mm to about 1000 mm onto a substrate.
2. The method of claim 1 , wherein the well comprises at least one of a polyethylene, polyethylene terephthalate, polypropylene, polymethacrylate, polyvinyl chloride, polystyrene, polyolefin, polycarbonate, polyurethane, starch-derived plastic, and cyclic olefin copolymer.
3. The method of claim 2 , wherein the well comprises cyclic olefin copolymer.
4. The method of claim 1 , wherein the well has a contact angle of about 5° to about 50°.
5. The method of claim 2 , wherein the well comprises polyolefin, and wherein the well has a surface energy from about 33 to about 45 dynes/cm.
6. The method of claim 2 , wherein the well comprises a polyethylene or polypropylene, and wherein the well has a surface energy from about 35 to about 55 dynes/cm.
7. The method of claim 1 , wherein a wall of the well has an angle from about 90 degrees to about 60 degrees.
8. The method of claim 1 , wherein the substrate comprises a soft material, a firm cushion material, a mat material, or a hard surface.
9. The method of claim 8 , wherein the substrate comprises a hard surface, and wherein the hard surface is stainless steel.
10. The method of claim 1 , wherein the solid composition comprises at least one of a bulking agent, an enzyme, an oligonucleotide, a buffer, a salt, nucleoside triphosphates, deoxynucleoside triphosphates, dATP, dGTP, dCTP, dTTP, dUTP, a capture probe, a hydrolysis probe, an RNase inhibitor, a chelating agent, a detergent, a polymerase, a pharmaceutically active agent, and reagents sufficient for performing a nucleic acid synthesis or amplification assay.
11. The method of claim 10 , wherein the solid composition comprises a bulking agent, and wherein the bulking agent comprises at least one of sucrose, mannitol, glycine, hydroxyethyl starch, raffinose, trehalose, and a saccharide.
12. The method of claim 1 , wherein the plasma treatment comprises a cold cathode discharge, hollow cathode discharge, DC-induced discharge, radio frequency (RF)-induced discharge, corona discharge, glow discharge, or charged particle beam.
13. The method of claim 1 , wherein the solid composition remains substantially attached when no visible part or less than about 10% or about 5% by volume of the solid composition becomes loose.