Composition and method of DNA marking elastomeric material
Methods of incorporating coded DNA into elastomeric materials and compositions thereof are claimed. Methods of recovering information from elastomeric materials with coded DNA and authenticating silicone objects with coded DNA are also claimed.
1. A method of authenticating a conductive silicone-metal composite gasket, the method comprising:
mixing a DNA taggant with conductive solid surface metal microspheres under conditions appropriate for the DNA taggant to adhere to the exterior surface of said metal microspheres to form DNA tagged conductive metal microspheres;
mixing the DNA tagged conductive metal microspheres with a quantity of silver-plated copper filled silicone to create DNA tagged silver-plated copper filled silicone;
molding the DNA tagged silver-plated copper filled silicone;
curing the DNA tagged silver-plated copper filled silicone to form a conductive silicone-metal composite gasket comprising said DNA tagged conductive metal microspheres, wherein the physical, conductive and electromagnetic shielding properties of the silicone-metal composite gasket formed from the DNA tagged silver-plated copper filled silicone are identical to said properties of a conductive silicone-metal composite gasket manufactured from non-DNA tagged silver-plated copper filled silicone;
placing a sample of said silicone-metal composite gasket formed from the DNA tagged silver-plated copper filled silicone into a polymerase chain reaction (PCR) vessel thereby performing in situ PCR to amplify the DNA taggant contained within the silicone-metal composite gasket; and
detecting the DNA taggant via capillary electrophoresis.
2. The method of claim 1 , wherein the DNA tagged silver-plated copper filled silicone is cured at temperatures equal to or greater than about 150° C.
3. The method of claim 1 , wherein the DNA tagged silver-plated copper filled silicone is cured by press curing and post curing.
4. The method of claim 1 , wherein the ratio of the DNA tagged conductive metal microspheres to the silver-plated copper filled silicone is between about 1:1 to about 10:1.
5. The method of claim 1 , wherein the conductive solid surface metal microspheres comprise silver-plated copper.
6. The method according to claim 1 , wherein the DNA taggant is mixed with an additive to form a treated DNA taggant, wherein the additive is methylated polyethylene glycol or un-methylated polyethylene glycol.
7. The method according to claim 1 , wherein the DNA taggant is mixed with an additive to form a treated DNA taggant, wherein the additive is selected from the group consisting of a polyol, a diol, a glycol, a starch and a pyrrolidone.