Patent
Provided are bioactive compositions and metabolites derived from Bacillus and particularly Bacillus megaterium cultures responsible for controlling pests as well as their methods of use for controlling pests. Further provided are pesticidal Bacillus megaterium strains.
1. A method for decreasing nematode infestation in a plant comprising the step of
applying to the plant and/or seeds thereof and/or substrate used for growing said plant an effective amount of a whole cell broth collected from Bacillus megaterium strain M018 (NRRL Accession No. B-50770) fermentation, wherein said Bacillus megaterium strain M018 comprises a 16S rRNA sequence as set forth in SEQ ID NO. 6;
and
optionally another substance, wherein said another substance is a pesticide and/or plant growth promoting agent.
2. The method of claim 1 , wherein the bacterium is in the form of a supernatant, filtrate, or cell fraction collected from said fermentation.
3. The method of claim 1 , wherein the pesticide comprises one or more single-site or multi-site anti-fungal agents.
4. The method of claim 1 , wherein the plant growth promoting agent comprises one or more synthetic or organic fertilizers and/or plant growth promoting microorganisms.
5. The method of claim 1 , further comprising the step of
applying 4-phenylbutanoic acid to the plant and/or seeds and/or substrate thereof.