Chimeric double-stranded nucleic acid
View Patent ↗A method of reducing the level of a transcription product in a cell comprising contacting with the cell a composition comprising a double-stranded nucleic acid complex comprising a first nucleic acid strand annealed to a second nucleic acid strand, wherein: (i) the first nucleic acid strand hybridizes to the transcription product and comprises (a) a region consisting of at least 4 consecutive nucleotides that are recognized by RNase H when the strand is hybridized to the transcription product, (b) one or more nucleotide analogs located on 5′ terminal side of the region, (c) one or more nucleotide analogs located on 3′ terminal side of the region and (d) a total number of nucleotides and nucleotide analogs ranging from 8 to 35 nucleotides and (ii) the second nucleic acid strand comprises (a) nucleotides and optionally nucleotide analogs and (b) at least 4 consecutive RNA nucleotides.
1. A composition comprising a double-stranded nucleic acid complex comprising a first nucleic acid strand annealed to a second nucleic acid strand, wherein:
(i) the first nucleic acid strand is 12 to 25 nucleotides in length, and hybridizes to a target transcription product, wherein the first nucleic acid strand comprises:
(a) a region consisting of at least 4 consecutive DNA nucleotides that are recognized by RNase H when the strand is hybridized to the target transcription product, wherein the at least 4 consecutive DNA nucleotides may be modified or unmodified;
(b) one or more nucleotide analogs located at the 5′ end of the region; and
(c) one or more nucleotide analogs located at the 3′ end of the region; and
(ii) the second nucleic acid strand is 12 to 31 nucleotides in length, and comprises:
(a) at least 4 consecutive RNA nucleotides; and
(b) a 5′ wing region of one or more modified nucleotides, nucleotide analogs and/or modified nucleotide analogs located 5′ to the at least 4 consecutive RNA nucleotides, and/or a 3′ wing region of one or more modified nucleotides, nucleotide analogs and/or modified nucleotide analogs located 3′ to the at least 4 consecutive RNA nucleotides, wherein the at least 4 consecutive RNA nucleotides can be cleaved by RNase H when the second nucleic acid strand is annealed with the first nucleic acid strand, and wherein the second nucleic acid strand further comprises a functional moiety having a function selected from a labeling function, a purification function, and a targeted delivery function.
2. The composition according to claim 1 , wherein the total number of nucleotides and nucleotide analogs in the first nucleic acid strand and the total number of nucleotides and nucleotide analogs in the second nucleic acid strand are the same.
3. The composition according to claim 1 , wherein the total number of nucleotides and nucleotide analogs in the first nucleic acid strand and the total number of nucleotides and nucleotide analogs in the second nucleic acid strand are different.
4. The composition according to claim 1 , wherein the nucleotide analogs in the first nucleic acid strand are bridged nucleotides.
5. The composition according to claim 1 , wherein at least one of the nucleotides and the nucleotide analogs in the first nucleic acid strand is phosphorothioated.
6. The composition according to claim 1 , wherein the second nucleic acid strand comprises one or more phosphorothioated nucleotides located 5′ and/or 3′ to the at least 4 consecutive RNA nucleotides.
7. The composition according to claim 1 , wherein the functional moiety is a molecule selected from the group consisting of a lipid, a glycolipid, a glyceride, a sugar, a peptide, and a protein.
8. The composition according to claim 1 , wherein the functional moiety is a lipid selected from the group consisting of a fatty acid, a lipid-soluble vitamin, a glycolipid, and a glyceride.
9. The composition according to claim 1 , wherein the functional moiety is a molecule selected from the group consisting of cholesterol, a tocopherol, a tocotrienol, glucose, sucrose, acylcarnitine, acyl-CoA, and an antibody.
10. The composition according to claim 1 , wherein at least one of the nucleotide analogs of the first nucleic acid strand or the second nucleic acid strand is a molecule selected from the group consisting of a hexitol nucleic acid (HNA), a cyclohexane nucleic acid (CeNA), a peptide nucleic acid (PNA), a glycol nucleic acid (GNA), a threose nucleic acid (TNA), a morpholino nucleic acid, a tricyclo-DNA (tcDNA), a 2′-O-methylated nucleic acid, a 2′-O-methoxyethylated (2′-MOE) nucleic acid, a 2′-O-aminopropylated (2′-AP) nucleic acid, a 2′-fluorinated nucleic acid, a 2′-F-arabinonucleic acid (2′-FANA), and a bridged nucleic acid (BNA).
11. The composition according to claim 10 , wherein the BNA is a molecule selected from the group consisting of α-L-methyleneoxy (4′-CH 2 —O-2′) BNA, β-D-methyleneoxy (4′-CH 2 —O-2′) BNA, ethyleneoxy (4′-CH 2 -2-O-2′) BNA, β-D-thio (4′-CH 2 —S-2′) BNA, aminooxy (4′-CH 2 —O—N(R 3 )-2′) BNA, oxyamino (4′-CH 2 —N(R 3 )—O-2′) BNA, 2′,4-BNA-COC, 3′-amino-2′,4′-BNA, 5′-methyl BNA, 4′-CH(CH 3 )—O-2′-BNA, 4′-CH(CH 2 OCH 3 )—O-2′-BNA, amide (4′-C(O)—N(H)-2′) BNA, and amide (4′-C(O)—N(Me)-2′) BNA.