Systems and methods for biological analysis
Provided herein are devices and methods suitable for sequencing, amplifying, analyzing, and performing sample preparation procedures for nucleic acids and other biomolecules.
1. A method for detecting at least a portion of a biological molecule, comprising:
(a) providing an array of sensors, wherein a sensor of said array of sensors comprises a transmitter electrode and a receiver electrode, which transmitter electrode or receiver electrode is coupled to at least another sensor of said array of sensors;
(b) directing a solution containing or suspected of containing said biological molecule to said array of sensors under conditions sufficient to bring said biological molecule to said sensor;
(c) using said transmitter electrode and said receiver electrode to measure a change in impedance in an electrical flow path comprising said at least said portion of said biological molecule; and
(d) using said change in impedance to detect said at least said portion of said biological molecule.
2. The method of claim 1 , wherein said electrical flow path comprises a double layer comprising said at least said portion of said biological molecule.
3. The method of claim 2 , wherein in (c), said at least said portion of said biological molecule is in said double layer.
4. The method of claim 1 , wherein subsequent to (b), said biological molecule interacts with another biological molecule adjacent to said sensor.
5. The method of claim 4 , wherein said another biological molecule is an aptamer, and wherein said biological molecule interacting with said aptamer generates said change in impedance.
6. The method of claim 4 , wherein said another biological molecule is coupled to a support, and wherein said support is in said electrical flow path.
7. The method of claim 1 , wherein said sensor and said at least another sensor are individually addressable.
8. The method of claim 1 , further comprising directing a plurality of particles to said array of sensors, wherein a particle of said plurality of particles is coupled to said biological molecule or another biological molecule configured to interact with said biological molecule, and wherein in (c), said particle is associated with said electrical flow path.
9. The method of claim 8 , wherein said particle is electrically coupled to a double layer comprising said at least said portion of said biological molecule or said another biological molecule.
10. The method of claim 8 , wherein said transmitter electrode and said receiver electrode are electrically isolated in the absence of said particle positioned adjacent thereto.
11. The method of claim 8 , wherein said change in impedance is across (i) said particle or (ii) a fluid environment comprising said particle.
12. The method of claim 8 , further comprising using a magnetic field to position said particle within said electrical flow path.
13. The method of claim 8 , further comprising using an electric field to position said particle within said electrical flow path.
14. The method of claim 8 , further comprising binding said biological molecule to an aptamer coupled to a catalytic unit, wherein binding of said biological molecule to said aptamer releases said catalytic unit into said solution, and wherein, upon release of said catalytic unit, said catalytic unit interacts with a nucleic acid molecule coupled to said particle.
15. The method of claim 14 , wherein said nucleic acid molecule is hybridized to a primer, and wherein said catalytic unit is a polymerase that extends said primer.
16. The method of claim 1 , wherein said biological molecule is a protein.
17. The method of claim 1 , wherein said biological molecule is a nucleic acid.
18. A method for detecting at least a portion of a biological molecule, comprising:
(a) providing an array of sensors, wherein a sensor of said array of sensors comprises a transmitter electrode and a receiver electrode, which transmitter electrode or receiver electrode is coupled to at least another sensor of said array of sensors;
(b) directing a solution containing or suspected of containing said biological molecule to said array of sensors under conditions sufficient to bring said biological molecule to said sensor;
(c) using said transmitter electrode and said receiver electrode to measure one or more signals that are indicative of a change in impedance in an electrical flow path comprising said at least said portion of said biological molecule, wherein said electrical flow path is between said transmitter electrode and said receiver electrode; and
(d) using said one or more signals to detect said at least said portion of said biological molecule.
19. The method of claim 1 , wherein said electrical flow path comprises said biological molecule.
20. The method of claim 1 , wherein said transmitter electrode or receiver electrode is shared with said at least another sensor.
21. The method of claim 18 , wherein said transmitter electrode or receiver electrode is shared with said at least another sensor.
22. The method of claim 18 , wherein said electrical flow path comprises a double layer comprising said at least said portion of said biological molecule.
23. The method of claim 22 , wherein in (c), said at least said portion of said biological molecule is in said double layer.
24. The method of claim 18 , wherein said sensor and said at least another sensor are individually addressable.
25. The method of claim 18 , further comprising directing a plurality of particles to said array of sensors, wherein a particle of said plurality of particles is coupled to said biological molecule or another biological molecule configured to interact with said biological molecule, and wherein in (c), said particle is associated with said electrical flow path.